CAJ | 학술논문

报道了先前克隆的柔红霉素产生菌天蓝淡红链霉菌(Streptomyces coeruleorubidus)dauW基因的过表达和阻断对柔红霉素产量的影响.通过原生质体融合将dauW表达质粒导入天蓝淡红链霉菌DM,所得重组菌DM-W1的柔红霉素产量无明显提高;而通过大肠杆菌-链霉菌接合转移获得的发生同源重组单交换的dauW阻断突变株DM-W2,柔红霉素产量达(192.0±61.6)μg/ml,且遗传稳定性好.试验结果表明,对dauW基因的阻断突变能明显提高柔红霉素的发酵单位.
보도료선전극륭적유홍매소산생균천람담홍련매균(Streptomyces coeruleorubidus)dauW기인적과표체화조단대유홍매소산량적영향.통과원생질체융합장dauW표체질립도입천람담홍련매균DM,소득중조균DM-W1적유홍매소산량무명현제고;이통과대장간균-련매균접합전이획득적발생동원중조단교환적dauW조단돌변주DM-W2,유홍매소산량체(192.0±61.6)μg/ml,차유전은정성호.시험결과표명,대dauW기인적조단돌변능명현제고유홍매소적발효단위.
A previously characterized gene dauW was studied for its influence on daunorubicin production. A dauW expression plasmid pYG766 was constructed and then transformed into daunorubicin producer, S. coeruleorubidus DM, via protoplast fusion. But the recombinant strain DM-Wl has no obvious daunorubicin production enhancement. dauW was disrupted by single-crossover and a mutant DM-W2 was obtained via E.coli-Streptomyces conjugation. Daunorubicin production in DM-W2 was (192.0±61.6)μg/ml. The result indicated that genetic manipulation of dauW in S. coeruleorubidus DM might contribute to productivity of daunorubicin.