中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2008年
4期
253-255
,共3页
胰腺炎,急性坏死性%NF-κB%受体,甲状旁腺激素%低钙血症
胰腺炎,急性壞死性%NF-κB%受體,甲狀徬腺激素%低鈣血癥
이선염,급성배사성%NF-κB%수체,갑상방선격소%저개혈증
Pancreatitis,acute necrotizing%NF-κB%Receptor,parathyroid hormone%Hypocalcacmia
目的 探讨NF-κB对ANP大鼠甲状旁腺激素受体(PTH1R)表达及低钙血症的影响.方法 将雄性SD大鼠24只按完全随机法分为对照组、ANP组和四氢化吡咯二硫代氨基甲酸脂预处理组(PDTC组),各8只.以5%牛磺脱氧胆酸钠逆行胰胆管注射建立ANP模型.观察6 h后各组血清钙浓度以及胰腺组织病理改变,蛋白免疫印渍法检测肾脏和骨组织NF-κB、PTH1R蛋白表达,RT-PCR检测肾脏和骨组织FTH1R mRNA表达.结果 对照组、ANP组和PDTC组血钙浓度分别为(2.31±0.03)mmol/L、(2.01±0.03)mmol/L和(2.12±0.05)mmol/L,3组间差异显著(P<0.05).ANP组肾脏和骨组织的NF-κB蛋白表达量分别为1.53±0.08和0.47±0.19;PDTC组分别为0.61±0.13和0.36±0.06,两组差异显著(P<0.05).ANP组肾脏和骨组织PTH1R mRNA的表达量分别为0.27±0.08和0.29±0.06,PTH1R蛋白表达分别为0.87±0.07和0.31±0.09;PDTC组分别为0.57±0.27和0.37±0.11,1.13±0.17和0.68±0.15,两组差异显著(P<0.05).ANP组NF-κB活性较对照组明显升高,PTH1R表达明显下降;与ANP组比较,PDTC组NF-κB活性降低,血清钙水平明显升高,PTH1R的表达上调(P<0.05).结论 NF-κB可能通过间接下调组织PTH1R的表达,致血清钙显著下降.PDTC对改善ANP低钙血症有一定意义.
目的 探討NF-κB對ANP大鼠甲狀徬腺激素受體(PTH1R)錶達及低鈣血癥的影響.方法 將雄性SD大鼠24隻按完全隨機法分為對照組、ANP組和四氫化吡咯二硫代氨基甲痠脂預處理組(PDTC組),各8隻.以5%牛磺脫氧膽痠鈉逆行胰膽管註射建立ANP模型.觀察6 h後各組血清鈣濃度以及胰腺組織病理改變,蛋白免疫印漬法檢測腎髒和骨組織NF-κB、PTH1R蛋白錶達,RT-PCR檢測腎髒和骨組織FTH1R mRNA錶達.結果 對照組、ANP組和PDTC組血鈣濃度分彆為(2.31±0.03)mmol/L、(2.01±0.03)mmol/L和(2.12±0.05)mmol/L,3組間差異顯著(P<0.05).ANP組腎髒和骨組織的NF-κB蛋白錶達量分彆為1.53±0.08和0.47±0.19;PDTC組分彆為0.61±0.13和0.36±0.06,兩組差異顯著(P<0.05).ANP組腎髒和骨組織PTH1R mRNA的錶達量分彆為0.27±0.08和0.29±0.06,PTH1R蛋白錶達分彆為0.87±0.07和0.31±0.09;PDTC組分彆為0.57±0.27和0.37±0.11,1.13±0.17和0.68±0.15,兩組差異顯著(P<0.05).ANP組NF-κB活性較對照組明顯升高,PTH1R錶達明顯下降;與ANP組比較,PDTC組NF-κB活性降低,血清鈣水平明顯升高,PTH1R的錶達上調(P<0.05).結論 NF-κB可能通過間接下調組織PTH1R的錶達,緻血清鈣顯著下降.PDTC對改善ANP低鈣血癥有一定意義.
목적 탐토NF-κB대ANP대서갑상방선격소수체(PTH1R)표체급저개혈증적영향.방법 장웅성SD대서24지안완전수궤법분위대조조、ANP조화사경화필각이류대안기갑산지예처리조(PDTC조),각8지.이5%우광탈양담산납역행이담관주사건립ANP모형.관찰6 h후각조혈청개농도이급이선조직병리개변,단백면역인지법검측신장화골조직NF-κB、PTH1R단백표체,RT-PCR검측신장화골조직FTH1R mRNA표체.결과 대조조、ANP조화PDTC조혈개농도분별위(2.31±0.03)mmol/L、(2.01±0.03)mmol/L화(2.12±0.05)mmol/L,3조간차이현저(P<0.05).ANP조신장화골조직적NF-κB단백표체량분별위1.53±0.08화0.47±0.19;PDTC조분별위0.61±0.13화0.36±0.06,량조차이현저(P<0.05).ANP조신장화골조직PTH1R mRNA적표체량분별위0.27±0.08화0.29±0.06,PTH1R단백표체분별위0.87±0.07화0.31±0.09;PDTC조분별위0.57±0.27화0.37±0.11,1.13±0.17화0.68±0.15,량조차이현저(P<0.05).ANP조NF-κB활성교대조조명현승고,PTH1R표체명현하강;여ANP조비교,PDTC조NF-κB활성강저,혈청개수평명현승고,PTH1R적표체상조(P<0.05).결론 NF-κB가능통과간접하조조직PTH1R적표체,치혈청개현저하강.PDTC대개선ANP저개혈증유일정의의.
Objective To investigate the effect of nuclear factor-kappa B(NF-κB) on the expression of parathyroid hormone receptor (PTH1R) and serum calcium level in rats with acute necrotizing pancreatitis (ANP) rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham operation group (SO), ANP group, pyrrolidine dithiocarbamat (PDTC, an inhibitor of NF-κB) pretreated ANP group (PDTC), with 8 rats each group. ANP model was induced by injecting 5% sodium taurodeoxyebolate into biliary pancreatic duct. Serum calcium was measured and the expression of NF-κB was determined by western blot and the expression of PTH1R was examined by RT-PCR plus western blot 6 h after ANP induction. Results The NF-κB proteins of bone and kidney tissues in ANP group were 1.53±0.08 and 0.47±0.19, respectively; in PDTC group they were 0.61±0.13 and 0.36±0.06 with significant difference (P<0.05). The PTH1R mRNA expressions of bone and kidney in ANP group were 0.27±0.08 and 0.29±0.06, respectively; the PTH1R proteins expressions were 0.87±0.07 and 0.31±0.09, respectively. The corresponding values in PDTC group were 0.57±0.27 and 0.37±0.11, 1.13±0.17 and 0.68±0.15, respectively, with significant difference (P<0.05). The activity of NF-κB increased in ANP group, the expressions of PTHI R decreased significantly. Compared with ANP group, the activity of NF-κB decreased and the levels of PTH1R and serum calcium increased in PDTC group (P<0.05). Conclusions NF-κB may indirectly down-regulate the expression of PTH1R, which resulting into the hypocalcaemia of ANP. PDTC may alleviate hypocalcaemia in ANP.
