CAJ | 학술논문

目的探讨孕酮减轻创伤性脑水肿的机制.方法建立雄性大鼠额叶脑挫裂伤模型并予孕酮干预治疗.免疫组织化学法检测脑组织星形胶质细胞AQP4表达的变化;伊文氏蓝法测定脑组织血脑屏障通透性的变化.结果经孕酮干预治疗,脑组织含水量在各时相点均下降(P＜0.05).脑挫裂伤后24、72、120 h时间段伤灶及其周边脑组织星形胶质细胞AQP4阳性细胞计数下降(P＜0.05);伤后6 h和24 h EB含量明显下降(P<0.01).结论孕酮减轻创伤性脑水肿的作用,可能与它降低外伤后脑组织AQP4表达水平和减轻伤后早期血脑屏障通透性有关.
목적 탐토잉동감경창상성뇌수종적궤제.방법 건립웅성대서액협뇌좌렬상모형병여잉동간예치료.면역조직화학법검측뇌조직성형효질세포AQP4표체적변화;이문씨람법측정뇌조직혈뇌병장통투성적변화.결과 경잉동간예치료,뇌조직함수량재각시상점균하강(P＜0.05).뇌좌렬상후24、72、120 h시간단상조급기주변뇌조직성형효질세포AQP4양성세포계수하강(P＜0.05);상후6 h화24 h EB함량명현하강(P<0.01).결론 잉동감경창상성뇌수종적작용,가능여타강저외상후뇌조직AQP4표체수평화감경상후조기혈뇌병장통투성유관.
Objective To discuss the mechanism of progesterone that soften brain water content in traumatic brain edema in rats. Methods The models of focal lobe contusion and laceration of brain were made on the male rats treated by the progesterone following injury. Immunohistochemical method was used to assess the expression of aquaporin-4 (AQP4). Evan's Blue method was used to detect the permeability of blood-brain barrier. Results Treated by the progesteron, the brain water content was significantly decreased, and the lower expression of AQP4 took place on astrocytes of the contusion and peri-contusion of the brain tissue after 24 h,72h ,and 120h . The content of EB was decreased at 6 h and 24 h post-injury. Conclusions Progesterone can soften the traumatic brain water content, which may be associated with the attenuation of AQP4 in frontal lobe contusion following traumatic brain injury ( TBI) and progesterone can protect the blood-brain barrier at early time after TBI.