中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
6期
752-755
,共4页
糖尿病,1型/遗传学%1-烷基-2-乙酰甘油磷酸胆碱酯酶/遗传学%突变
糖尿病,1型/遺傳學%1-烷基-2-乙酰甘油燐痠膽堿酯酶/遺傳學%突變
당뇨병,1형/유전학%1-완기-2-을선감유린산담감지매/유전학%돌변
Diabetes mellitus,type 1/GE%1-alkyl-2 -acetylglycerophosphocholine esterase/GE%Mutation
目的 观察初发1型糖尿病(T1DM)病人血浆血小板活化因子乙酰水解酶(PAF-AH)的活性以及PAF-AH基因G994T与T1DM的相关性.方法 选择T1DM病人155例,对照组138例,测定血浆PAF-AH活性,采用PCR法测定PAF-AH基因G994T基因型.结果 T1DM患者的血浆PAF-AH活性为(20.64±6.23)nmol/(min·ml);健康对照者血浆PAF-AH活性为(28.56±4.11)nmol/(min·ml),两组比较差异有统计学意义(P<0.05).TIDM组与对照组相比,G994T位点GT基因型差异无统计学意义(8.4% VS 7.2%,P>0.05).结论 初发T1DM患者的血浆PAF-AH活性降低.PAF-AH基因G994T突变位点可能与1型糖尿病无相关性.
目的 觀察初髮1型糖尿病(T1DM)病人血漿血小闆活化因子乙酰水解酶(PAF-AH)的活性以及PAF-AH基因G994T與T1DM的相關性.方法 選擇T1DM病人155例,對照組138例,測定血漿PAF-AH活性,採用PCR法測定PAF-AH基因G994T基因型.結果 T1DM患者的血漿PAF-AH活性為(20.64±6.23)nmol/(min·ml);健康對照者血漿PAF-AH活性為(28.56±4.11)nmol/(min·ml),兩組比較差異有統計學意義(P<0.05).TIDM組與對照組相比,G994T位點GT基因型差異無統計學意義(8.4% VS 7.2%,P>0.05).結論 初髮T1DM患者的血漿PAF-AH活性降低.PAF-AH基因G994T突變位點可能與1型糖尿病無相關性.
목적 관찰초발1형당뇨병(T1DM)병인혈장혈소판활화인자을선수해매(PAF-AH)적활성이급PAF-AH기인G994T여T1DM적상관성.방법 선택T1DM병인155례,대조조138례,측정혈장PAF-AH활성,채용PCR법측정PAF-AH기인G994T기인형.결과 T1DM환자적혈장PAF-AH활성위(20.64±6.23)nmol/(min·ml);건강대조자혈장PAF-AH활성위(28.56±4.11)nmol/(min·ml),량조비교차이유통계학의의(P<0.05).TIDM조여대조조상비,G994T위점GT기인형차이무통계학의의(8.4% VS 7.2%,P>0.05).결론 초발T1DM환자적혈장PAF-AH활성강저.PAF-AH기인G994T돌변위점가능여1형당뇨병무상관성.
Objective To investigate the plasma PAF-AH level in initialed type 1 diabetes patients and the association between the PAF-AH gene polymorphism and type 1 diabetes mellitus. Methods 155 initialed type 1 diabetes patients and 138 controls were selected in this study. Plasma PAF-AH was determined by PAF-AH Assay kit, and the PAE-AH gene G994t genotypes were detected by PCR. Results The activity of plasma PAF-AH in the patients with type 1 diabetes was significantly lower than that in the controls[ (20.64 ± 6.23)nmol/(min · ml) vs (28.56 ± 4. 11)nmol/(min · ml), P <0.05). Compared with control group, the frequencies of the GT genotype on G994T polymorphism between type 1 diabetes patients and controls were not significantly different(8.4% vs 7. 2%, P >0. 05). Conclusion The activity
of plasma PAF-AH in the patients with type 1 diabetes was significantly lower than controls. The G994T polymorphism of PAF-AH gene was not related to type 1 diabetes mellitus.