中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
7期
742-746
,共5页
靳雅丽%刘斌%江朝强%徐琳%郭黎红%林大庆%林洁明%张维森%郑家强%Thomas GN
靳雅麗%劉斌%江朝彊%徐琳%郭黎紅%林大慶%林潔明%張維森%鄭傢彊%Thomas GN
근아려%류빈%강조강%서림%곽려홍%림대경%림길명%장유삼%정가강%Thomas GN
左心室肥厚%ENPP1基因%多态性
左心室肥厚%ENPP1基因%多態性
좌심실비후%ENPP1기인%다태성
Left ventricular hypertrophy%Ectonucleotide pyrophosphatase/phosphodiesterasel%Polymorphism
目的 研究中老年人核苷酸外焦磷酸酶/磷酸二酯酶1(ENPP1)基因多态位点rs1409181与左心室肥厚的关系.方法 从"广州生物库队列-CVD"项目中的1996名研究对象随机选出390名年龄≥50岁的中老年广州市居民,收集其个人资料与病史、体格检查并测定血清空腹血糖、血脂水平;应用彩色多普勒超声诊断仪,同步连接心电图,测定左心室舒张末期内径(LVIDD)、舒张期室间隔厚度(IVSD)、舒张期后壁厚度(LVPWD).根据Devereux公式计算左心室质量(LVM)和左心室质量指数(LVMI)以判断左心室肥厚.应用荧光定量PCR方法检测ENPP1基因rs1409181位点的基因型.结果 ENPP1基因GG、CG和CC 3个基因型组左心室肥厚的比率分别为21.5%、28.2%和37.5%.与GG基因型相比,CG和CC基因型经调整包括血压和胰岛素抵抗指数在内等多种混杂因素后,对左心室肥厚的比值比(OR)分别为1.39(95%CI:0.78～2.50)和2.36(95%CI:1.21～4.60),趋势检验P=0.01.结论 ENPP1基因多态位点rs1409181与左心室肥厚显著相关.
目的 研究中老年人覈苷痠外焦燐痠酶/燐痠二酯酶1(ENPP1)基因多態位點rs1409181與左心室肥厚的關繫.方法 從"廣州生物庫隊列-CVD"項目中的1996名研究對象隨機選齣390名年齡≥50歲的中老年廣州市居民,收集其箇人資料與病史、體格檢查併測定血清空腹血糖、血脂水平;應用綵色多普勒超聲診斷儀,同步連接心電圖,測定左心室舒張末期內徑(LVIDD)、舒張期室間隔厚度(IVSD)、舒張期後壁厚度(LVPWD).根據Devereux公式計算左心室質量(LVM)和左心室質量指數(LVMI)以判斷左心室肥厚.應用熒光定量PCR方法檢測ENPP1基因rs1409181位點的基因型.結果 ENPP1基因GG、CG和CC 3箇基因型組左心室肥厚的比率分彆為21.5%、28.2%和37.5%.與GG基因型相比,CG和CC基因型經調整包括血壓和胰島素牴抗指數在內等多種混雜因素後,對左心室肥厚的比值比(OR)分彆為1.39(95%CI:0.78～2.50)和2.36(95%CI:1.21～4.60),趨勢檢驗P=0.01.結論 ENPP1基因多態位點rs1409181與左心室肥厚顯著相關.
목적 연구중노년인핵감산외초린산매/린산이지매1(ENPP1)기인다태위점rs1409181여좌심실비후적관계.방법 종"엄주생물고대렬-CVD"항목중적1996명연구대상수궤선출390명년령≥50세적중노년엄주시거민,수집기개인자료여병사、체격검사병측정혈청공복혈당、혈지수평;응용채색다보륵초성진단의,동보련접심전도,측정좌심실서장말기내경(LVIDD)、서장기실간격후도(IVSD)、서장기후벽후도(LVPWD).근거Devereux공식계산좌심실질량(LVM)화좌심실질량지수(LVMI)이판단좌심실비후.응용형광정량PCR방법검측ENPP1기인rs1409181위점적기인형.결과 ENPP1기인GG、CG화CC 3개기인형조좌심실비후적비솔분별위21.5%、28.2%화37.5%.여GG기인형상비,CG화CC기인형경조정포괄혈압화이도소저항지수재내등다충혼잡인소후,대좌심실비후적비치비(OR)분별위1.39(95%CI:0.78～2.50)화2.36(95%CI:1.21～4.60),추세검험P=0.01.결론 ENPP1기인다태위점rs1409181여좌심실비후현저상관.
Objective To examine the association between genetic polymorphism of rs1409181 in ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) and left ventricular hypertrophy (LVH) among older Chinese in Guangzhou. Methods 390 subjects aged ≥50 years were randomly selected from the Guangzhou Biobank Cohort Study-CVD. Information on personal history, blood pressure, fasting plasma glucose and lipids were collected. Color Doppler ultrasound was used to measure the indicators of LVH, including left ventricular internal diastolic diameter (LVIDD) , thickness of the interventricular septum diastolic wall (IVSD) and the posterior wall diastolic diameter (LVPWD). LVIDD was calculated using Devereux ventricular mass (LVM)equation while the Left ventricular mass index (LVMI) equation was used to estimate LVH. The genotype of rs1409181 was determined by Taqman SNP genotyping kits using the ABI 7900HT real time PCR system. Results In the GG, CG and CC genotype groups, the proportions of LVH were 21.5%, 28.2% and 37.5% respectively. Compared with GG, the adjusted odds ratios (95% confidence interval) for the LVH were 1.39(0.78-2.50) and 2.36(1.21-4.60) for CG genotype and CC genotype of ENPP1 respectively (P for trend=0.01). Conclusion Polymorphism of ENPP1 gene rs1409181 was associated with LVH in the older Chinese people in Guangzhou.
