中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
3期
129-133
,共5页
李鹏%肖小娥%徐泉%郭正团
李鵬%肖小娥%徐泉%郭正糰
리붕%초소아%서천%곽정단
血管瘤%模型,动物%细胞系
血管瘤%模型,動物%細胞繫
혈관류%모형,동물%세포계
Hemangioma%Mode,animal%Cell line
目的 建立婴幼儿血管瘤源性血管内皮细胞系(hemangioma-derived endothelial cell line,HemEC)及其动物模型.方法 采用组织块法进行HemEC体外培养,制作HemEC生长曲线,免疫组化法行Ⅷ因子相关抗原鉴定,流式细胞仪鉴定HemEC的血管内皮生长因子受体2(vascular endothelial growth factor receptor2,VEGFR-2)的表达,分析HemEC染色体核型,采用异体移植的方法将HemEC接种于裸鼠皮下,观察肿瘤的生长情况,进行组织病理学检查.结果 培养的细胞生长活跃,传到第16代时细胞增殖增快,自发永生化,共传代培养70代以上,体外培养时间1年,冻存、复苏多次,细胞仍生长良好,具有HemEC的形态学特征,细胞群体倍增时间约为22 h,Ⅷ因子和VEGFR-2均阳性表达,HemEC的染色体表现为非正常的二倍体特征,染色体数介于二倍体和三倍体之间,将其命名为XPTS-1.该婴幼儿血管瘤动物模型的组织病理学特征与婴幼儿血管瘤的组织病理学特征基本一致.结论 本研究建立的HemEC自发转化、永生化,其形态学特征和生物学特性符合HemEC的特性,既具有一般转化细胞系的饱和密度生长、接触性抑制、停泊依赖性生长等特性,又具有致瘤性等特性,符合婴幼儿血管瘤组织病理学特性,该模型的组织病理学特征与婴幼儿血管瘤的组织病理学特征基本一致.
目的 建立嬰幼兒血管瘤源性血管內皮細胞繫(hemangioma-derived endothelial cell line,HemEC)及其動物模型.方法 採用組織塊法進行HemEC體外培養,製作HemEC生長麯線,免疫組化法行Ⅷ因子相關抗原鑒定,流式細胞儀鑒定HemEC的血管內皮生長因子受體2(vascular endothelial growth factor receptor2,VEGFR-2)的錶達,分析HemEC染色體覈型,採用異體移植的方法將HemEC接種于裸鼠皮下,觀察腫瘤的生長情況,進行組織病理學檢查.結果 培養的細胞生長活躍,傳到第16代時細胞增殖增快,自髮永生化,共傳代培養70代以上,體外培養時間1年,凍存、複囌多次,細胞仍生長良好,具有HemEC的形態學特徵,細胞群體倍增時間約為22 h,Ⅷ因子和VEGFR-2均暘性錶達,HemEC的染色體錶現為非正常的二倍體特徵,染色體數介于二倍體和三倍體之間,將其命名為XPTS-1.該嬰幼兒血管瘤動物模型的組織病理學特徵與嬰幼兒血管瘤的組織病理學特徵基本一緻.結論 本研究建立的HemEC自髮轉化、永生化,其形態學特徵和生物學特性符閤HemEC的特性,既具有一般轉化細胞繫的飽和密度生長、接觸性抑製、停泊依賴性生長等特性,又具有緻瘤性等特性,符閤嬰幼兒血管瘤組織病理學特性,該模型的組織病理學特徵與嬰幼兒血管瘤的組織病理學特徵基本一緻.
목적 건립영유인혈관류원성혈관내피세포계(hemangioma-derived endothelial cell line,HemEC)급기동물모형.방법 채용조직괴법진행HemEC체외배양,제작HemEC생장곡선,면역조화법행Ⅷ인자상관항원감정,류식세포의감정HemEC적혈관내피생장인자수체2(vascular endothelial growth factor receptor2,VEGFR-2)적표체,분석HemEC염색체핵형,채용이체이식적방법장HemEC접충우라서피하,관찰종류적생장정황,진행조직병이학검사.결과 배양적세포생장활약,전도제16대시세포증식증쾌,자발영생화,공전대배양70대이상,체외배양시간1년,동존、복소다차,세포잉생장량호,구유HemEC적형태학특정,세포군체배증시간약위22 h,Ⅷ인자화VEGFR-2균양성표체,HemEC적염색체표현위비정상적이배체특정,염색체수개우이배체화삼배체지간,장기명명위XPTS-1.해영유인혈관류동물모형적조직병이학특정여영유인혈관류적조직병이학특정기본일치.결론 본연구건립적HemEC자발전화、영생화,기형태학특정화생물학특성부합HemEC적특성,기구유일반전화세포계적포화밀도생장、접촉성억제、정박의뢰성생장등특성,우구유치류성등특성,부합영유인혈관류조직병이학특성,해모형적조직병이학특정여영유인혈관류적조직병이학특정기본일치.
Objective To establish an immortalized human infancy hemangioma-derived endothelial cell line (HemEC) and animal model of human infancy hemangioma. Methods Hemangioma-derived endothelial cells from specimen of human infancy hemangioma were cultured in vitro and monocloed, and then its growth curve was made, karyomorphism of chromosome analyzed, morphologic characteristics observe,factor Ⅷ related antigen identified by immunohistochemical method. Vascular endothelial growth factor receptor 2(VEGFR-2) was detected by flow cytometry. HemEC were inoculated subcutaneously in athymicmouse to establish animal model of infancy hemangioma. The animal model was observed closely and its pathological characteristic was also studied. Results The cultural cells grew active, and immortalized spontaneously when they were subcultured on sixteenth generation. This cell line was cultivated for more than 70 times within one year and in good condition after freezing and resuscitating once and again, and had the morphologic character of HemEC. The cell population doubling time was 22 h. Factor Ⅷ and VEGFR-2 were expressed positively. Karyo type analysis of the cell line showed abnormal diploid with the modal chromosomal number varying between diploid and triploid. The cell line was then named XPTS-1. The animal model of infancy hemangioma was successfully established and its character of histopathology was similar with that of infancy hemangioma. Conclusions The cell line of HemEC was successfully established and immortalized spontaneously, and had the morphologic and biological character of HemEC. The animal model of infancy hemangioma was successfully established and showed the character of histopathology similar with that of infancy hemangioma.
