CAJ | 학술논문

目的探讨BMI-1基因在自血病中的表达及意义.方法应用反转录-聚合酶链式反应(RT-PCR)技术,检测BMI-1 mRNA在慢性粒细胞白血病(CML)细胞株(K562),慢性粒细胞白血病(CML)、慢性淋巴细胞白血病(CLL)、急性白血病(AL)患者和对照组的骨髓单个核细胞(BMMNC)中的表达及临床意义.结果 BMI-1基因在对照组中无表达;K562细胞株中旱阳性表达;CML组中的阳性率为15.4%,其中CML慢性期(CML-CP)与CML急变期(CML-AP)组阳性率比较差异有统计学意义(P＜0.05);CLL组中无表达;AL组中阳性率为47.1%.结论 BMI-1基凶在白血病中存在较高的表达,且与疾病的转归相关,可能为白血病的靶向治疗提供新的位点.
목적 탐토BMI-1기인재자혈병중적표체급의의.방법 응용반전록-취합매련식반응(RT-PCR)기술,검측BMI-1 mRNA재만성립세포백혈병(CML)세포주(K562),만성립세포백혈병(CML)、만성림파세포백혈병(CLL)、급성백혈병(AL)환자화대조조적골수단개핵세포(BMMNC)중적표체급림상의의.결과 BMI-1기인재대조조중무표체;K562세포주중한양성표체;CML조중적양성솔위15.4%,기중CML만성기(CML-CP)여CML급변기(CML-AP)조양성솔비교차이유통계학의의(P＜0.05);CLL조중무표체;AL조중양성솔위47.1%.결론 BMI-1기흉재백혈병중존재교고적표체,차여질병적전귀상관,가능위백혈병적파향치료제공신적위점.
Objective To investigate the expression and its significance of BMI-1 gene in leukemia patients.Methods BMI-1 gene level was assessed by reverse transcription polymerase chain reaction (RT-PCR)in acute leukemia patients,chronic myeloid leukemia(CML)patients,chronic lymphoblastic leukemia(CLL)patients,and bone marrow mononuclear cells(BMMNC)as normal controls,as well as leukemia cell line K562.The expression of BMI-1 gene in leukemia and the relationship was explored.Results The expression of BMI-1 gene was positive in leukemia cell line K562.but negative in 10 controls as well as in CLL.The BMI-1 gene was expressed in 15.4% of CML patients and 47.1% of AL patients.The positive expression of BMI-1 gene in CML-CR was significandy lower than that in CML-BP (P＜0.05).Conclusion BMI-1 gene was over expressed in leukemia patients,and may be related to curative effect of the disease.It may be used for leukemia treatment targeting the provision of new sites.