CAJ | 학술논문

目的应用犬骨髓基质细胞片层构建组织工程骨,为临床提供组织工程骨来源.方法分离培养传代犬骨髓基质细胞(bone marrow stromal cell,BMSC).将经成骨诱导的第3代BMSC接种于温度反应性培养皿中,制备BMSC细胞片层.制备犬同种异体脱钙骨基质(decalcification bone matrixes,DBM).实验用16只犬分为4组,每组4只,采用自身对照.将复合体BMSC片层-人重组骨形态生成蛋白2( rhBMP-2) -BMSC-DBM植入犬左侧背阔肌肌筋膜下为实验侧,同法右侧植入DBM-rhBMP-2-BMSC为对照侧.术后4、8、12、16周取材行组织学观察,评价体内异位成骨的情况.结果实验侧成骨优于对照侧,成骨面积实验侧＞对照侧,两侧差异有统计学意义(P＜0.05).术后16周,实验侧板层骨连接成片,可见骨单位,骨髓腔内可见红骨髓.结论 BMSC细胞片层可促进功能性组织工程骨的形成.
목적 응용견골수기질세포편층구건조직공정골,위림상제공조직공정골래원.방법 분리배양전대견골수기질세포(bone marrow stromal cell,BMSC).장경성골유도적제3대BMSC접충우온도반응성배양명중,제비BMSC세포편층.제비견동충이체탈개골기질(decalcification bone matrixes,DBM).실험용16지견분위4조,매조4지,채용자신대조.장복합체BMSC편층-인중조골형태생성단백2( rhBMP-2) -BMSC-DBM식입견좌측배활기기근막하위실험측,동법우측식입DBM-rhBMP-2-BMSC위대조측.술후4、8、12、16주취재행조직학관찰,평개체내이위성골적정황.결과 실험측성골우우대조측,성골면적실험측＞대조측,량측차이유통계학의의(P＜0.05).술후16주,실험측판층골련접성편,가견골단위,골수강내가견홍골수.결론 BMSC세포편층가촉진공능성조직공정골적형성.
Objective To construct tissue engineering bone with bone marrow stromal cell(BMSC)sheets of dogs.Methods BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis.Allogeneic dogs decalcification bone matrixes(DBM) were prepared.Sixteen dogs were divided into 4 groups.The MSC cell sheets-rhBMP2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the thBMP-2-BMSC-DBM were implanted in the right side as the control.Ectopic bone formation in vivo was evaluated by histological examination 4,8,12,16 weeks after operation.Results The osteogenesis in the experimental group was better than that in the control group.New bone area in the experimental side was larger than that in the control group,and the difference was significant ( P ＜ 0.05 ).After 16 weeks,lamellar bone was connected into a film in the experimental group.Haversian system and red bone marrow could be seen.Conclusions BMSC cell sheets could promote the bone formation of functional tissueengineered bone.