CAJ | 학술논문

目的分析不同剂量60Co γ射线部分照射人离体血对淋巴细胞染色体畸变形成的影响.方法用0～8 Gy(剂量率为0.35 Gy/min)60Coγ射线在37 ℃条件下照射3份离体健康人外周血标本,以0.5∶1的比例与同一供血者的未受照血混合,120 min后进行培养、制片,显微镜下分析染色体畸变(双着丝粒+着丝粒环)的变化,借此进行剂量估算.结果各组的双着丝粒体+着丝粒环和总畸变,以及断片和单体断裂均随着剂量的增加而增加.用双着丝粒+着丝粒环进行的剂量估算,0.5～2 Gy组大于照射剂量的1/3,4～8 Gy组均接近照射剂量的1/3.结论染色体畸变可以作为估算非均匀照射的生物学指标之一.
목적 분석불동제량60Co γ사선부분조사인리체혈대림파세포염색체기변형성적영향.방법 용0～8 Gy(제량솔위0.35 Gy/min)60Coγ사선재37 ℃조건하조사3빈리체건강인외주혈표본,이0.5∶1적비례여동일공혈자적미수조혈혼합,120 min후진행배양、제편,현미경하분석염색체기변(쌍착사립+착사립배)적변화,차차진행제량고산.결과 각조적쌍착사립체+착사립배화총기변,이급단편화단체단렬균수착제량적증가이증가.용쌍착사립+착사립배진행적제량고산,0.5～2 Gy조대우조사제량적1/3,4～8 Gy조균접근조사제량적1/3.결론 염색체기변가이작위고산비균균조사적생물학지표지일.
Objective To investigate the effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro.Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃ ,and then mixed with the unirradiated blood samples of the Microscopy was used to observe the chromosome aberration double ( centromere + centromere) and the biological dose was estimated thereby.ResultsThe amounts of double centromere + centromere were increased along with the dose of irradiation in all groups.The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy,and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy.Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation.