CAJ | 학술논문

采用随机扩增多态DNA(RAPD)标记对瓣蕊唐松草(Thalictrum petaloideum L.)(毛莨科)11个居群246个体的遗传多样性及居群遗传结构进行了检测.20个随机引物扩增出125个用于分析的条带.结果表明:瓣蕊唐松草在物种水平上具有极高的遗传多样性,多态条带比率(PPB)为96%,Nei的基因多样度(h)为0.3502,Shannon多样性指数(I)为0.519 9;居群间分化比较明显,基因分化系数(GsT)为0.351 1,分子方差分析(AMOVA)表明居群间遗传变异占总遗传变异的38.88%;根据条带频率与平均分类距离和Nei遗传距离分别进行UPGMA聚类,结果基本一致,并且反映出与该种地理分布有一定相关性;长江流域分布的两个居群表现出较高的相似性,并与北方分布的各居群构成独立的两支,显示出这两个居群的特殊性,从而对该物种独特分布区成因是由于冰退回迁造成的观点提供了一定的支持.
채용수궤확증다태DNA(RAPD)표기대판예당송초(Thalictrum petaloideum L.)(모랑과)11개거군246개체적유전다양성급거군유전결구진행료검측.20개수궤인물확증출125개용우분석적조대.결과표명:판예당송초재물충수평상구유겁고적유전다양성,다태조대비솔(PPB)위96%,Nei적기인다양도(h)위0.3502,Shannon다양성지수(I)위0.519 9;거군간분화비교명현,기인분화계수(GsT)위0.351 1,분자방차분석(AMOVA)표명거군간유전변이점총유전변이적38.88%;근거조대빈솔여평균분류거리화Nei유전거리분별진행UPGMA취류,결과기본일치,병차반영출여해충지리분포유일정상관성;장강류역분포적량개거군표현출교고적상사성,병여북방분포적각거군구성독립적량지,현시출저량개거군적특수성,종이대해물충독특분포구성인시유우빙퇴회천조성적관점제공료일정적지지.
Random amplified polymorphic DNA (RAPD) method was applied to assess genetic variation and population structure of Thalictrum petaloideum L. (Ranunculaceae). Two hundred and forty-six individuals from 11 populations of the species were investigated by RAPD profiles. Twenty selected RAPD primers generated 125 bands, in which 120 were polymorphic. The results revealed a high level of genetic variation (percentage of polymorphic bands (PPB) was 96%, Nei's gene diversity (h) was 0.350 2 and shannon's information index (1) was 0.519 9 at the species level). The differentiation among the populations was high (GsT = 0.351 1)in this species. Result of analyzing of molecular variance (AMOVA) showed that 38.88% of genetic variance was found among the populations. Positive correlation with r= 0.194 5 (P= 0.000 2)was found between genetic distance and geographic distance among populations. Two populations distributed in the drainage basin of Yangtz River affined genetically and formed one clade and the rest nine populations formed the other clade in both unweighted pair-group method using arithmetic average (UPGMA)trees made by two different methods. It was very clear that these two populations were very special, and must be closely related in history, despite the fact that they now share quite weak link to the rest populations through gene communication.