中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1569-1571
,共3页
刘玉峰%苏泽轩%赖彩永%袁道彰%唐晖%罗云%程云华%丁泓文%陈洁%都兴华%石利平
劉玉峰%囌澤軒%賴綵永%袁道彰%唐暉%囉雲%程雲華%丁泓文%陳潔%都興華%石利平
류옥봉%소택헌%뢰채영%원도창%당휘%라운%정운화%정홍문%진길%도흥화%석리평
EN2%同源异型盒蛋白%前列腺癌
EN2%同源異型盒蛋白%前列腺癌
EN2%동원이형합단백%전렬선암
Engrail-2%Homeobox protein%Prostate cancer
目的 探讨同源异型盒基因EN-2在不同前列腺癌细胞及人正常前列腺上皮细胞中的表达差异及意义.方法 采用逆转录-聚合酶链反应( RT-PCR)和免疫荧光法检测前列腺癌PC3、DU145和LNCAP细胞以及人正常前列腺上皮细胞RWPE-1中EN2mRNA和蛋白的表达,采用酶联免疫吸附试验( ELISA)法检测上述细胞培养液中EN2蛋白的含量变化.结果 RT-PCR和ELISA检测结果显示:EN2 mRNA和蛋白水平在激素依赖性前列腺癌细胞LNCAP(1.65±0.19、8.74±0.34)和激素非依赖性前列腺癌细胞PC3中(1.08±0.12、3.07±0.24)及DU145 (1.15 ±0.53、2.86±0.26)均高于人正常前列腺上皮细胞(0.42 ±0.11、0.86 ±0.99) (P ＜0.05),其中LNCAP细胞中EN2mRNA和蛋白表达水平高于其余两种前列腺癌细胞(P＜0.05).结论 EN2在前列腺上皮细胞中呈低表达,在各前列腺癌细胞株中表达增高,并为前列腺癌细胞所分泌.EN2的表达可能与前列腺癌发生发展有关.
目的 探討同源異型盒基因EN-2在不同前列腺癌細胞及人正常前列腺上皮細胞中的錶達差異及意義.方法 採用逆轉錄-聚閤酶鏈反應( RT-PCR)和免疫熒光法檢測前列腺癌PC3、DU145和LNCAP細胞以及人正常前列腺上皮細胞RWPE-1中EN2mRNA和蛋白的錶達,採用酶聯免疫吸附試驗( ELISA)法檢測上述細胞培養液中EN2蛋白的含量變化.結果 RT-PCR和ELISA檢測結果顯示:EN2 mRNA和蛋白水平在激素依賴性前列腺癌細胞LNCAP(1.65±0.19、8.74±0.34)和激素非依賴性前列腺癌細胞PC3中(1.08±0.12、3.07±0.24)及DU145 (1.15 ±0.53、2.86±0.26)均高于人正常前列腺上皮細胞(0.42 ±0.11、0.86 ±0.99) (P ＜0.05),其中LNCAP細胞中EN2mRNA和蛋白錶達水平高于其餘兩種前列腺癌細胞(P＜0.05).結論 EN2在前列腺上皮細胞中呈低錶達,在各前列腺癌細胞株中錶達增高,併為前列腺癌細胞所分泌.EN2的錶達可能與前列腺癌髮生髮展有關.
목적 탐토동원이형합기인EN-2재불동전렬선암세포급인정상전렬선상피세포중적표체차이급의의.방법 채용역전록-취합매련반응( RT-PCR)화면역형광법검측전렬선암PC3、DU145화LNCAP세포이급인정상전렬선상피세포RWPE-1중EN2mRNA화단백적표체,채용매련면역흡부시험( ELISA)법검측상술세포배양액중EN2단백적함량변화.결과 RT-PCR화ELISA검측결과현시:EN2 mRNA화단백수평재격소의뢰성전렬선암세포LNCAP(1.65±0.19、8.74±0.34)화격소비의뢰성전렬선암세포PC3중(1.08±0.12、3.07±0.24)급DU145 (1.15 ±0.53、2.86±0.26)균고우인정상전렬선상피세포(0.42 ±0.11、0.86 ±0.99) (P ＜0.05),기중LNCAP세포중EN2mRNA화단백표체수평고우기여량충전렬선암세포(P＜0.05).결론 EN2재전렬선상피세포중정저표체,재각전렬선암세포주중표체증고,병위전렬선암세포소분비.EN2적표체가능여전렬선암발생발전유관.
Objective To investigate the expression of Engrail-2 (EN2) and its significance in different prostate cancer cell lines and normal human prostate epithelial cell line.Methods The expression of EN2 was detected in prostate cancer cell lines (PC3,DU145,LNCAP) and normal prostate epithelial cell line RWPE-1 by using reverse transcription-polymerase chain reaction (RT-PCR),enzyme linked immunosorbent assay (ELISA) and immunofluorescence.Results The EN2 mRNA and protein expression levels in Pca cell lines ( LNCAP,PC3,DU145 ) were 1.65 ± 0.19/8.74 ± 0.34,1.08 ± 0.12/3.07 ±0.24,and 1.15 ±0.53/2.86± 0.26 respectively,which was significantly higher than in normal prostate epithelial cell line RWPE-1 (0.42 ± 0.11/0.86 ± 0.99 ) ( P ＜ 0.05 ).The EN2 level was higher in LNCAP cells than in PC3 and DU145 cells (P ＜ 0.05).Conclusion The expression of EN2 in 3 prostate cancer cell lines is higher than in RWPE-1 and EN2 protein can be secreted by prostate cancer cells.It is important for the further study on the EN2 potential roles in the occurrence and development of prostate carcinoma.
