CAJ | 학술논문

目的探讨蒙汉民族人群人类白细胞抗原(HLA)-DQB1基因多态性与支气管哮喘的相关性.方法 2007年3月至2009年3月采用序列特异性引物聚合酶链反应(PCR-SSP)法对汉族哮喘患者50例、蒙古族哮喘患者68例进行HLA-DQB1等位基因频率的检测,并分别与50例健康汉族和54例健康蒙古族进行比较.结果汉族支气管哮喘HLA-DQB1 0602基因频率高于汉族健康对照组(OR=6.163,P＜0.01);蒙古族支气管哮喘者HLA-DQB1 0603/0608基因频率低于健康蒙古族对照组(OR=0.199,P＜0.05);蒙古族支气管哮喘HLA-DQB1 0301/4基因频率高于健康蒙古族对照组(OR=2.074,P＜0.05);蒙古族支气管哮喘HLA-DQB1 DQB1 0301/4基因频率高于汉族哮喘组(OR=2.482,P=0.05);健康蒙古族组HLA-DQB1 0602基因频率高于健康汉族对照组(OR=3.341,P＜0.05);健康蒙古族组HLA-DQB1 0402基因频率低于健康汉族对照组(OR=0.209,P＜0.05).结论 HLA-DQB1 0603/0608基因可能是蒙古族哮喘患者的保护基因,HLA-DQB1 0301/4基因可能是蒙古族哮喘患者的易感基因,HLA-DQB1 0602基因可能是汉族支气管哮喘患者的易感基因.
목적 탐토몽한민족인군인류백세포항원(HLA)-DQB1기인다태성여지기관효천적상관성.방법 2007년3월지2009년3월채용서렬특이성인물취합매련반응(PCR-SSP)법대한족효천환자50례、몽고족효천환자68례진행HLA-DQB1등위기인빈솔적검측,병분별여50례건강한족화54례건강몽고족진행비교.결과 한족지기관효천HLA-DQB1 0602기인빈솔고우한족건강대조조(OR=6.163,P＜0.01);몽고족지기관효천자HLA-DQB1 0603/0608기인빈솔저우건강몽고족대조조(OR=0.199,P＜0.05);몽고족지기관효천HLA-DQB1 0301/4기인빈솔고우건강몽고족대조조(OR=2.074,P＜0.05);몽고족지기관효천HLA-DQB1 DQB1 0301/4기인빈솔고우한족효천조(OR=2.482,P=0.05);건강몽고족조HLA-DQB1 0602기인빈솔고우건강한족대조조(OR=3.341,P＜0.05);건강몽고족조HLA-DQB1 0402기인빈솔저우건강한족대조조(OR=0.209,P＜0.05).결론 HLA-DQB1 0603/0608기인가능시몽고족효천환자적보호기인,HLA-DQB1 0301/4기인가능시몽고족효천환자적역감기인,HLA-DQB1 0602기인가능시한족지기관효천환자적역감기인.
Objective To investigate association between human leukocyte antigen DQB1 (HLADQB1 ) gene polymorphisms and bronchial asthma among Mongolian and Han nationalities. Methods Sequence-specific primer polymerase chain reaction (PCR-SSP) was used to detect frequencies of HLA DQB1 genotypes and alleles in 50 cases of Han and 68 Mongolian asthmatic patients, and 50 Han and 54 Mongolian healthy controls, respectively. Difference in gene frequencies between the two nationalities was estimated by odds ratio (OR) and chi-square test. Results Frequency of the HLA-DQB1 0602 allele was significantly higher in Han patients with bronchial asthma than that in healthy Han nationality (OR = 6.163,P ＜0.01 ). Frequency of the HLA-DQB1 0603/0608 allele decreased in Mongolian asthmatic patients, as compared to that in healthy Mongolians ( OR = 0.199, P ＜ 0.05 ). Frequency of the HLA-DQB1 0301/4 allele was significantly higher in Mongolian asthmatic patients as compared to that in healthy Mongolians ( OR =2.074,P ＜0.05). Frequency of the HLA-DQB1 0301/4 allele was significantly higher in Mongolian than that in Han asthmatic patients ( OR = 2.482 ,P =0.05). Frequency of the HLA- DQB1 0602 allele was significantly higher in healthy Mongolians than that in healthy Han nationality ( OR = 3.341, P ＜ 0.05 ), in contrast, frequency of the HLA-DQB1 0402 allele was significantly lower in healthy Mongolians than that in healthy Han nationality ( OR = 0.209, P ＜ 0.05 ). Conclusions The HLA-DQB1 0603/0608 allele is possibly a protective gene and the HLA-DQB1 0301/4 allele a susceptible gene for bronchial asthma in Mongolians, and the HLA-DQB1 0602 allele is possibly a susceptible gene for bronchial asthma in Han nationlity.