CAJ | 학술논문

目的通过观察吗替麦考酚酯(MMF)对小鼠辅助性T淋巴细胞17(TH 17细胞)分化和增殖的影响,探讨MMF的免疫抑制作用及其机制.方法采用随机数字表法将小鼠分为MMF组与对照组,每组8只.MMF组小鼠每天给予MMF 40 mg·kg-1·d-1灌胃,对照组小鼠每天给予等体积生理盐水灌胃.3周后取小鼠外周血和脾脏,采用流式细胞术检测小鼠外周血和脾细胞中TH17细胞和CD4+CD25+调节性T淋巴细胞(Treg细胞)的比例,并计算出TH 17细胞与Treg细胞的比值;采用酶联免疫吸附试验法分别检测两组小鼠血清中白细胞介素(IL)-17和IL-23的浓度.结果 MMF组外周血和脾细胞中TH17细胞比例分别为(1.95±0.08)％和(2.42±0.06)％,对照组分别为(3.19±0.07)％和(4.21±0.25)％,两组比较,差异均有统计学意义(P＜0.05).MMF组外周血和脾细胞中TH 17细胞与Treg细胞的比值均显著低于对照组(P＜0.05).MMF组小鼠血清IL-17水平明显低于对照组(P＜0.05),而血清IL-23水平高于对照组(P＜0.05).结论 MMF能够明显抑制小鼠体内TH17细胞的分化与增殖,降低TH 17细胞与Treg细胞的比值,减少IL-17的分泌,有利于诱导免疫耐受.
목적 통과관찰마체맥고분지(MMF)대소서보조성T림파세포17(TH 17세포)분화화증식적영향,탐토MMF적면역억제작용급기궤제.방법 채용수궤수자표법장소서분위MMF조여대조조,매조8지.MMF조소서매천급여MMF 40 mg·kg-1·d-1관위,대조조소서매천급여등체적생리염수관위.3주후취소서외주혈화비장,채용류식세포술검측소서외주혈화비세포중TH17세포화CD4+CD25+조절성T림파세포(Treg세포)적비례,병계산출TH 17세포여Treg세포적비치;채용매련면역흡부시험법분별검측량조소서혈청중백세포개소(IL)-17화IL-23적농도.결과 MMF조외주혈화비세포중TH17세포비례분별위(1.95±0.08)％화(2.42±0.06)％,대조조분별위(3.19±0.07)％화(4.21±0.25)％,량조비교,차이균유통계학의의(P＜0.05).MMF조외주혈화비세포중TH 17세포여Treg세포적비치균현저저우대조조(P＜0.05).MMF조소서혈청IL-17수평명현저우대조조(P＜0.05),이혈청IL-23수평고우대조조(P＜0.05).결론 MMF능구명현억제소서체내TH17세포적분화여증식,강저TH 17세포여Treg세포적비치,감소IL-17적분비,유리우유도면역내수.
Objective To observe the effects of mycophenolate mofetil (MMF) on the differentiation and proliferation of Helper T cells 17 (TH 17),so as to reveal its role and the possible mechanism in inducing immunological suppression.Methods Sixteen Balb/c mice of SPF level aged 8 weeks were randomly divided into two groups:MMF group and control group,with 8 mice in each group.In MMF group,the mice received intragastric administration of MMF (40 mg·kg-1· day-1 ),and those in control group received intragastric administration of identical volumetric saline every day.After three weeks,peripheral blood was collected and spleen cells were prepared.Flow cytometry was used to determine the proportions of CD4+ TH 17 and CD4+ CD25+ Tregs,then the ratio of TH 17/Tregs was calculated,and the concentrations of interleukin-1 7 (IL-1 7) and interleukin-23 (IL-23) in serum were measured by ELISA.Results The proportion of CD4+ TH 17 in the peripheral blood and spleen was (1.95 ± 0.08) and (2.42 ± 0.06) in MMF group,and (3.19 ± 0.07)％ and (4.21 ± 0.25)％ in control group,respectively.There were significant differences between the two groups (P ＜0.05).Meanwhile,the ratio of TH 17/Tregs in MMF group,both in the peripheral blood and spleen,was significantly decreased as compared with the control group (P＜0.05).The concentration of IL-17 in MMF group was lower,but that of IL-23 in MMF group was higher than in the control group (P＜0.05).Conclusion MMF could obviously suppress the differentiation and proliferation of CD4+ TH 17 in vivo,reduce the ratio of TH17/Tregs and the IL-17 secretion,thus facilitate the induction of immune tolerance.