中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2002年
3期
422-424
,共3页
姜荣龙%冯筱榕%郭亚兵%卢桥生%侯金林%骆抗先%富宁
薑榮龍%馮篠榕%郭亞兵%盧橋生%侯金林%駱抗先%富寧
강영룡%풍소용%곽아병%로교생%후금림%락항선%부저
慢性乙型肝炎病毒感染%白细胞介素-4%干扰素-γ%转化生长因子-β
慢性乙型肝炎病毒感染%白細胞介素-4%榦擾素-γ%轉化生長因子-β
만성을형간염병독감염%백세포개소-4%간우소-γ%전화생장인자-β
chronic hepatitis B virus infection * interleukin-4 * inetrferon-γ * transform growth factor-β
目的 了解慢性乙型肝炎病毒（HBV）感染者外周血中辅助性T细胞（Th）内干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）和转化生长因子-β（TGF-β）的表达情况,以测定Th1/Th2 /Th3细胞的百分数,探明Th细胞各极化群体在慢性HBV感染中的作用.方法 常规分离外周血单个核细胞（PBMC）,在佛波脂（PMA）、钙离子导入剂伊屋诺霉素Ionomycin、细胞内转运阻断剂莫能星Monensin的刺激下,采用流式细胞分析术（FACS）对慢性HBV感染者外周血单个Th细胞内IL-4、IFN-γ和TGF-β的表达进行分析.结果 正常对照组,2.3%-18.6%的CD4+T细胞为Th1细胞,0.9%-9.2%为Th2细胞；0.7%-7.1%的细胞仅表达TGF-β, 为Th3细胞.在慢性HBV感染者外周血单个CD4+T细胞中,以Th0细胞居多；而Th1细胞则随着慢性乙型肝炎肝脏炎症活动的加剧而明显增多,在炎症活动明显的慢性乙型肝炎,其Th1百分数明显高于炎症活动呈静止状态的慢性乙型肝炎；Th2细胞在慢性HBV感染的不同阶段则较为恒定(P>0.05),但明显高于对照组(P<0.05).Th3细胞的百分数则随着肝组织炎症活动的加剧,其比例也明显减少(P<0.05),在无症状携带者（AsC）组,Th3细胞的百分数远高于慢性乙型肝炎组及对照组(P<0.05) .Th3细胞重叠于Th2细胞之中.结论 Th1细胞类因子与炎症活动呈正相关,Th2细胞可能与HBV感染的慢性化相关联.而Th3细胞与Th2细胞协同发挥负性免疫调节作用,并可能与慢性HBV感染的免疫耐受状态相关联.
目的 瞭解慢性乙型肝炎病毒（HBV）感染者外週血中輔助性T細胞（Th）內榦擾素-γ（IFN-γ）、白細胞介素-4（IL-4）和轉化生長因子-β（TGF-β）的錶達情況,以測定Th1/Th2 /Th3細胞的百分數,探明Th細胞各極化群體在慢性HBV感染中的作用.方法 常規分離外週血單箇覈細胞（PBMC）,在彿波脂（PMA）、鈣離子導入劑伊屋諾黴素Ionomycin、細胞內轉運阻斷劑莫能星Monensin的刺激下,採用流式細胞分析術（FACS）對慢性HBV感染者外週血單箇Th細胞內IL-4、IFN-γ和TGF-β的錶達進行分析.結果 正常對照組,2.3%-18.6%的CD4+T細胞為Th1細胞,0.9%-9.2%為Th2細胞；0.7%-7.1%的細胞僅錶達TGF-β, 為Th3細胞.在慢性HBV感染者外週血單箇CD4+T細胞中,以Th0細胞居多；而Th1細胞則隨著慢性乙型肝炎肝髒炎癥活動的加劇而明顯增多,在炎癥活動明顯的慢性乙型肝炎,其Th1百分數明顯高于炎癥活動呈靜止狀態的慢性乙型肝炎；Th2細胞在慢性HBV感染的不同階段則較為恆定(P>0.05),但明顯高于對照組(P<0.05).Th3細胞的百分數則隨著肝組織炎癥活動的加劇,其比例也明顯減少(P<0.05),在無癥狀攜帶者（AsC）組,Th3細胞的百分數遠高于慢性乙型肝炎組及對照組(P<0.05) .Th3細胞重疊于Th2細胞之中.結論 Th1細胞類因子與炎癥活動呈正相關,Th2細胞可能與HBV感染的慢性化相關聯.而Th3細胞與Th2細胞協同髮揮負性免疫調節作用,併可能與慢性HBV感染的免疫耐受狀態相關聯.
목적 료해만성을형간염병독（HBV）감염자외주혈중보조성T세포（Th）내간우소-γ（IFN-γ）、백세포개소-4（IL-4）화전화생장인자-β（TGF-β）적표체정황,이측정Th1/Th2 /Th3세포적백분수,탐명Th세포각겁화군체재만성HBV감염중적작용.방법 상규분리외주혈단개핵세포（PBMC）,재불파지（PMA）、개리자도입제이옥낙매소Ionomycin、세포내전운조단제막능성Monensin적자격하,채용류식세포분석술（FACS）대만성HBV감염자외주혈단개Th세포내IL-4、IFN-γ화TGF-β적표체진행분석.결과 정상대조조,2.3%-18.6%적CD4+T세포위Th1세포,0.9%-9.2%위Th2세포；0.7%-7.1%적세포부표체TGF-β, 위Th3세포.재만성HBV감염자외주혈단개CD4+T세포중,이Th0세포거다；이Th1세포칙수착만성을형간염간장염증활동적가극이명현증다,재염증활동명현적만성을형간염,기Th1백분수명현고우염증활동정정지상태적만성을형간염；Th2세포재만성HBV감염적불동계단칙교위항정(P>0.05),단명현고우대조조(P<0.05).Th3세포적백분수칙수착간조직염증활동적가극,기비례야명현감소(P<0.05),재무증상휴대자（AsC）조,Th3세포적백분수원고우만성을형간염조급대조조(P<0.05) .Th3세포중첩우Th2세포지중.결론 Th1세포류인자여염증활동정정상관,Th2세포가능여HBV감염적만성화상관련.이Th3세포여Th2세포협동발휘부성면역조절작용,병가능여만성HBV감염적면역내수상태상관련.
Objective To investigate the compositions of Th1/Th2/Th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-γ (IFN-γ), and transform growth factor-β (TGF-β) in single CD4+ T cells isolated from peripheral blood mononuclear cells (PBMCs) and the role of polarized Th cell populations in chronic HBV-infection was discussed. Methods PBMCs from chronically infected HBV individuals were isolated, stimulated by PMA/Ionomycin/Monensin, and IL-4, IFN-γ and TGF-β production by CD4+ T cells was determined by using fluorescence activated cell sorter (FACS) analysis. Results The percentage of IFN-γ-producing T cells, IL-4-producing T cells and TGF-β-producing T cells ranged from 2.3%-18.6%, 1.1%-8.7% and 0.7%-7.1% respectively in CD4+ T cells from non-infected individuals. Most of CD4+ T cells from PBMCs in chronically infected HBV individuals were Th0 cells. The proportion of Th1 cells increased significantly with hepatic inflammatory activity, and in the active period of chronic hepatitis B infection were higher than those in the non-active period (P<0.05). Th2 cell percentage in CD4+ T cells from HBV-infected individuals did not differ significantly (P>0.05), but were higher than that from controls (P<0.05). Th3 cell percentage in CD4+ T cells from asymptomatic carrier (AsC) group was higher than that in the chronic hepatitis B (CHB) and control groups (P<0.05). Conclusions Th1 phenotype cytokines were positively correlated with hepatic inflammatory activity in chronic hepatitis B and Th2 cells may be associated with the persistence of HBV infection. Th3 cells cooperating with Th2 cells can negatively regulate immune responses and may be associated with the immune tolerant state of chronic HBV infection.
