中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
1期
75-77
,共3页
陈敏%马艳玲%楚承霞%邢辉%徐艳声%苏莹珍%杨颖%陈会超%罗红兵%贾曼红%陆林
陳敏%馬豔玲%楚承霞%邢輝%徐豔聲%囌瑩珍%楊穎%陳會超%囉紅兵%賈曼紅%陸林
진민%마염령%초승하%형휘%서염성%소형진%양영%진회초%라홍병%가만홍%륙림
艾滋病病毒%耐药警戒线检测%抗病毒治疗
艾滋病病毒%耐藥警戒線檢測%抗病毒治療
애자병병독%내약경계선검측%항병독치료
Human immunodeficiency virus%Drug resistance transmission threshold survey%Antiretroviral therapy
目的 了解2010年昆明市未经抗病毒治疗的HIV感染者中耐药株的传播水平.方法 根据WHO推荐的HIV耐药警戒线调查方法,对2010年1-8月收集的62份年龄＜25岁的HIV-1新发现感染者的血液样本进行HIV-1耐药基因型检测和耐药株传播水平分析.结果 62份样本中,54份完成耐药基因型鉴定.按pol基因区进行分型,其中53.2％的样本为CRF08 BC亚型,其他亚型包括CRF07_BC、CRF01_AE、C.根据采样时间顺序,前47份扩增阳性的样本用于耐药传播水平的分析,其中1份出现针对蛋白酶抑制剂类药物的耐药突变位点,耐药株流行率＜5％.结论 昆明市HIV耐药株尚处于低度流行水平,为控制耐药传播水平的上升,规范治疗及科学管理仍然是当前艾滋病患者治疗工作的重点.
目的 瞭解2010年昆明市未經抗病毒治療的HIV感染者中耐藥株的傳播水平.方法 根據WHO推薦的HIV耐藥警戒線調查方法,對2010年1-8月收集的62份年齡＜25歲的HIV-1新髮現感染者的血液樣本進行HIV-1耐藥基因型檢測和耐藥株傳播水平分析.結果 62份樣本中,54份完成耐藥基因型鑒定.按pol基因區進行分型,其中53.2％的樣本為CRF08 BC亞型,其他亞型包括CRF07_BC、CRF01_AE、C.根據採樣時間順序,前47份擴增暘性的樣本用于耐藥傳播水平的分析,其中1份齣現針對蛋白酶抑製劑類藥物的耐藥突變位點,耐藥株流行率＜5％.結論 昆明市HIV耐藥株尚處于低度流行水平,為控製耐藥傳播水平的上升,規範治療及科學管理仍然是噹前艾滋病患者治療工作的重點.
목적 료해2010년곤명시미경항병독치료적HIV감염자중내약주적전파수평.방법 근거WHO추천적HIV내약경계선조사방법,대2010년1-8월수집적62빈년령＜25세적HIV-1신발현감염자적혈액양본진행HIV-1내약기인형검측화내약주전파수평분석.결과 62빈양본중,54빈완성내약기인형감정.안pol기인구진행분형,기중53.2％적양본위CRF08 BC아형,기타아형포괄CRF07_BC、CRF01_AE、C.근거채양시간순서,전47빈확증양성적양본용우내약전파수평적분석,기중1빈출현침대단백매억제제류약물적내약돌변위점,내약주류행솔＜5％.결론 곤명시HIV내약주상처우저도류행수평,위공제내약전파수평적상승,규범치료급과학관리잉연시당전애자병환자치료공작적중점.
Objective To study the HIV drug resistance (HIVDR) transmission in Kunming city of Yunnan province in 2010.Methods Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS) set by WHO,62 plasma samples of recently reported HIV-infected individuals who were older than 25 years of age,were collected from January to August 2010.Genotyping of pol genetic mutations associated with HIVDR with reverse transcriptional PCR was performed and the prevalence of HIV-1 drug resistance transmission was evaluated.Results Of the 62 plasma samples,54 were successfully sequenced and genotyped on pol sequence.Based on the pol sequences,HIV subtypes including CRF08_BC ( 53.2％ ),CRF07_BC ( 25.5％ ),CRF01_AE ( 19.1％ )and C (2.1％) were identified.According to the time of sampling,the first 47 sequenced samples were used for drug resistance prevalence analysis.A protease inhibitor (PI) relative mutation was found in one sample.Based on the WHO standard,the prevalence of transmitted HIV-1 drug resistance was ＜5％.Conclusion HIV-1 drug resistant strains transmission was still catalogued as low prevalence level in Kunming.To prevent the increase of HIVDR prevalence,normative treatment and scientific management to AIDS patients seemed to be quite important.
