中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2008年
33期
18-21
,共4页
牙周炎%基质金属蛋白酶9%免疫印迹法%龈沟液
牙週炎%基質金屬蛋白酶9%免疫印跡法%齦溝液
아주염%기질금속단백매9%면역인적법%간구액
Periodontitis%Matrix metalloproteinase 9%Immunoblotting%Gingival crevicular fluid
目的 观察慢性牙周炎患者在牙周基础治疗前后龈沟液中基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制剂-1(TIMP-1)水平的变化.方法 采用滤纸条牙周袋内取样法提取15例慢性牙周炎患者在牙周基础治疗前以及基础治疗结束后1个月的龈沟液样本,同时提取15例牙周健康者的龈沟液样本,采用免疫印迹法检测MMP-9与TIMP-1水平.结果 慢性牙周炎患者治疗后龈沟液中MMP-9水平[(1.2±0.9)AU]较治疗前[(2.2±1.4)AU]显著下降,TIMP-1水平[(3.7±2.2)AU]较治疗前[(1.3±1.2)AU]显著升高,P<0.05;MMP-9、TIMP-1水平分别与牙周病临床参数呈显著正相关、显著负相关.结论 MMP-9、TIMP-1水平反映了牙周组织的破坏程度,可作为评价疗效的客观指标.
目的 觀察慢性牙週炎患者在牙週基礎治療前後齦溝液中基質金屬蛋白酶-9(MMP-9)、基質金屬蛋白酶組織抑製劑-1(TIMP-1)水平的變化.方法 採用濾紙條牙週袋內取樣法提取15例慢性牙週炎患者在牙週基礎治療前以及基礎治療結束後1箇月的齦溝液樣本,同時提取15例牙週健康者的齦溝液樣本,採用免疫印跡法檢測MMP-9與TIMP-1水平.結果 慢性牙週炎患者治療後齦溝液中MMP-9水平[(1.2±0.9)AU]較治療前[(2.2±1.4)AU]顯著下降,TIMP-1水平[(3.7±2.2)AU]較治療前[(1.3±1.2)AU]顯著升高,P<0.05;MMP-9、TIMP-1水平分彆與牙週病臨床參數呈顯著正相關、顯著負相關.結論 MMP-9、TIMP-1水平反映瞭牙週組織的破壞程度,可作為評價療效的客觀指標.
목적 관찰만성아주염환자재아주기출치료전후간구액중기질금속단백매-9(MMP-9)、기질금속단백매조직억제제-1(TIMP-1)수평적변화.방법 채용려지조아주대내취양법제취15례만성아주염환자재아주기출치료전이급기출치료결속후1개월적간구액양본,동시제취15례아주건강자적간구액양본,채용면역인적법검측MMP-9여TIMP-1수평.결과 만성아주염환자치료후간구액중MMP-9수평[(1.2±0.9)AU]교치료전[(2.2±1.4)AU]현저하강,TIMP-1수평[(3.7±2.2)AU]교치료전[(1.3±1.2)AU]현저승고,P<0.05;MMP-9、TIMP-1수평분별여아주병림상삼수정현저정상관、현저부상관.결론 MMP-9、TIMP-1수평반영료아주조직적파배정도,가작위평개료효적객관지표.
Objective To examine the changes of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase-1 (TIMP-1) in gingival crevicular fluid after conventional mechanical therapy of adult patients with chronic periodontitis. Methods Gingival crevicular fluid samples from 15 patients and 15 healthy subjects were taken with filter paper strips by intra-pocket method initially, respectively, 1 month after scaling and root planning. MMP-9,TIMP-1 levels were measured by immunoblotting. Results MMP-9 levels in patients were redueed significantly after treatment[(2.2±1.4)AU vs (1.2±0.9)AU ]. The clinical parameters were positive correlation with MMP-9 level. TIMP-1 levels in patients were increased significantly after treatment[(1.3±1.2)AU vs (3.7±2.2)AU]. The clinical parameters were negative torrelation with TIMP-1 level. Conclusion MMP-9, TIMP-1 levels can act as objective parameters to evaluate the effect of periodontal therapy.
