CAJ | 학술논문

目的研究5-氟尿嘧啶(5-fluorouracil,5-FU)缓释微粒对肝癌HepG2细胞的抑制作用.方法 MTT法检测5-FU缓释微粒、5-FU裸药和缓释微粒载体(聚L-乳酸)对人肝癌HepG2细胞的抑制作用,采用Hochest染色和流式细胞仪检测细胞凋亡.结果 5-Fu缓释微粒组细胞存活率随着时间的延长而降低,凋亡细胞数目也随之增加.5-FU裸药组第1天的抑制作用最强,有大量细胞凋亡,随着作用时间的延长,细胞存活率缓慢上升,第21、28天的细胞存活率高于5.FU缓释微粒组.缓释微粒载体组细胞凋亡均较少.3组比较差异有统计学意义(F=3163.52,128.47,P＜0.01).结论植入用5-FU缓释微粒可以在28 d内持续释放,并且以时间依赖性方式抑制肝癌HepG2细胞生长和诱导其凋亡增加,明显优于5-FU裸药.
목적 연구5-불뇨밀정(5-fluorouracil,5-FU)완석미립대간암HepG2세포적억제작용.방법 MTT법검측5-FU완석미립、5-FU라약화완석미립재체(취L-유산)대인간암HepG2세포적억제작용,채용Hochest염색화류식세포의검측세포조망.결과 5-Fu완석미립조세포존활솔수착시간적연장이강저,조망세포수목야수지증가.5-FU라약조제1천적억제작용최강,유대량세포조망,수착작용시간적연장,세포존활솔완만상승,제21、28천적세포존활솔고우5.FU완석미립조.완석미립재체조세포조망균교소.3조비교차이유통계학의의(F=3163.52,128.47,P＜0.01).결론 식입용5-FU완석미립가이재28 d내지속석방,병차이시간의뢰성방식억제간암HepG2세포생장화유도기조망증가,명현우우5-FU라약.
Objective To evaluate the inhibitory effects of sustained-release 5-fluorouracil(5-FU)micmparticles on the growth of HepG2 eels.Methods The inhibitory effects of sustained-releage 5-FU micropartides,5-FU microparticles and poly L-lactic acid on HepG2 cells were detected by MTT assay.The HepG2 cell apoptosis was demonstrated by flow cytomerry after Hoehest staining.Results The survival rate of HepG2 cells in sustained-release 5-FU microparticles group decreased as time passed by.The survival rate of HepG2 cells in 5-FU microparticles group wag the lowest on the first day,and then it increased gradually.The survival rates of HepG2 ceHs in 5-FU microparticles group on day 21 and 28 were hJigher than those in sustained-release 5-FU microparticles group.The survival rate of HepG2 cells in poly L-lactic acid group WSB higher than that in the other two groups.The difference upon survival rate among the 3 groups had statistical significance(F=3163.52,128.47.P＜0.01).Conclusions The sustained-release 5-FU micropartieles could keep on inhibiting tlle growth and inducing apoptosis of HepG2 cells in time-dependant manner.The inhibitory effect of sustained-release 5-FU is better than that of 5-FU microparticles.