CAJ | 학술논문

目的建立一种快速、简便、灵敏和特异的舌形虫病血清学诊断方法 .方法从人工感染尖吻蝮蛇舌状虫虫卵3个月的KM小鼠中分离尖吻蝮蛇舌状虫若虫,制备粗抗原,进行SDS-PAGE鉴定分析.用该粗抗原ELISA检测舌形虫病患者、不同寄生虫病患者和尖吻蝮蛇舌状虫实验感染小鼠血清特异性IgG抗体,其中舌形虫病患者血清I份,血吸虫病患者血清13份,囊尾蚴、并殖吸虫、肝吸虫和旋毛虫等病患者血清各5份,丝虫、裂头蚴病患者血清各1份,正常人血清10份以及舌形虫感染不同时间段的小鼠血清30份.结果舌形虫若虫粗抗原浓度为37.9 ms/ml,SDS-PAGE鉴定分析,可见4条清晰的条带,相对分子质量为16000～150000.舌形虫病患者和29份舌形虫感染小鼠血清检查结果均为阳性,其他寄生虫病患者血清检查结果均为阴性.结论初步建立了基于尖吻蝮蛇舌状虫若虫粗抗原检测舌形虫病的ELISA方法 ,为该病临床诊断、疾病预防控制及试剂盒的研制提供依据和奠定了基础.
목적 건립일충쾌속、간편、령민화특이적설형충병혈청학진단방법 .방법 종인공감염첨문복사설상충충란3개월적KM소서중분리첨문복사설상충약충,제비조항원,진행SDS-PAGE감정분석.용해조항원ELISA검측설형충병환자、불동기생충병환자화첨문복사설상충실험감염소서혈청특이성IgG항체,기중설형충병환자혈청I빈,혈흡충병환자혈청13빈,낭미유、병식흡충、간흡충화선모충등병환자혈청각5빈,사충、렬두유병환자혈청각1빈,정상인혈청10빈이급설형충감염불동시간단적소서혈청30빈.결과설형충약충조항원농도위37.9 ms/ml,SDS-PAGE감정분석,가견4조청석적조대,상대분자질량위16000～150000.설형충병환자화29빈설형충감염소서혈청검사결과균위양성,기타기생충병환자혈청검사결과균위음성.결론 초보건립료기우첨문복사설상충약충조항원검측설형충병적ELISA방법 ,위해병림상진단、질병예방공제급시제합적연제제공의거화전정료기출.
Objective To establish a rapid,simple,sensitive and specific serodiagnostie method for pentastomiosis.Methods Nymphs were isolated from KM mice infected with eggs ofArmillifer agkistrodontis,for 3 months.The soluble raw antigens were prepared from the nymphs,analysed by SDS-PAGE,and were used for detecting specific IgG antibodies in sera from patients with different parasitic diseases wherein 1 with pentastomiosis,13 with schistosomiasis,5 with cystieercasis,paragonimiasis,clonorchiasis and trichinosis,respectivelv.1 with filariasis,1 with plerocereoidosis,10 healthy persons and 30 KM mice infected with A.agkistrodontis at different time by ELISA.Results The concentration of soluble raw antigens Was 37.9 ms/m1.and 4 different bands about M,16000.150000 were visible on SDS-PAGE gels.The sera of pentastomiosis and 29 KM mice infected with A.agkistrodontis were positive,and the others were negative by ELISA detection.Conclusion EUSA WaS established for diagnosing pentastomiosis using soluble raw antigens of A.agkistrodontis nymphs.