中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
8期
515-517
,共3页
樊长晖%乔保平%文建国%祁园明%阮翘
樊長暉%喬保平%文建國%祁園明%阮翹
번장휘%교보평%문건국%기완명%원교
肾肿瘤%癌%疫苗%树突细胞
腎腫瘤%癌%疫苗%樹突細胞
신종류%암%역묘%수돌세포
Kidney noeplasms%Carcinoma%Vaccines%Dendritic cells
目的 探讨负载人肾癌细胞抗原肽制备树突状细胞(DC)疫苗体外杀伤肾癌细胞的作用.方法 利用细胞膜酸洗脱法获得人肾透明细胞癌细胞株786-0细胞表面抗原肽.外周血单个核细胞在体外经人粒细胞一巨噬细胞集落刺激因子,白细胞介素4和脂多糖诱导获得成熟的DC,并负载分离到的抗原肽制备DC疫苗.利用疫苗体外诱导出特异性细胞毒性T淋巴细胞(CTL)作为实验组.同时设置4个对照组,对照组1用未负载抗原肽的DC和单个核细胞混合培养,对照组2用单个核细胞进行培养,对照组3用抗原肽与单个核细胞混合培养,对照组4用未负载抗原肽的DC单独培养.4个对照组也加入同实验组相同的各种因子进行培养.51Cr释放法检测特异性CTL的杀伤活性.结果 疫苗诱导的特异性CTL对肾癌细胞的杀伤活性为(31.93±5.05)%,与对照组(5.88±2.26)%、(8.03±6.70)%、(9.70±2.09)%、(9.35±3.58)%相比差异有统计学意义(P<0.05).结论 负载抗原肽的DC疫苗体外试验有高效的抗肾癌细胞活性.
目的 探討負載人腎癌細胞抗原肽製備樹突狀細胞(DC)疫苗體外殺傷腎癌細胞的作用.方法 利用細胞膜痠洗脫法穫得人腎透明細胞癌細胞株786-0細胞錶麵抗原肽.外週血單箇覈細胞在體外經人粒細胞一巨噬細胞集落刺激因子,白細胞介素4和脂多糖誘導穫得成熟的DC,併負載分離到的抗原肽製備DC疫苗.利用疫苗體外誘導齣特異性細胞毒性T淋巴細胞(CTL)作為實驗組.同時設置4箇對照組,對照組1用未負載抗原肽的DC和單箇覈細胞混閤培養,對照組2用單箇覈細胞進行培養,對照組3用抗原肽與單箇覈細胞混閤培養,對照組4用未負載抗原肽的DC單獨培養.4箇對照組也加入同實驗組相同的各種因子進行培養.51Cr釋放法檢測特異性CTL的殺傷活性.結果 疫苗誘導的特異性CTL對腎癌細胞的殺傷活性為(31.93±5.05)%,與對照組(5.88±2.26)%、(8.03±6.70)%、(9.70±2.09)%、(9.35±3.58)%相比差異有統計學意義(P<0.05).結論 負載抗原肽的DC疫苗體外試驗有高效的抗腎癌細胞活性.
목적 탐토부재인신암세포항원태제비수돌상세포(DC)역묘체외살상신암세포적작용.방법 이용세포막산세탈법획득인신투명세포암세포주786-0세포표면항원태.외주혈단개핵세포재체외경인립세포일거서세포집락자격인자,백세포개소4화지다당유도획득성숙적DC,병부재분리도적항원태제비DC역묘.이용역묘체외유도출특이성세포독성T림파세포(CTL)작위실험조.동시설치4개대조조,대조조1용미부재항원태적DC화단개핵세포혼합배양,대조조2용단개핵세포진행배양,대조조3용항원태여단개핵세포혼합배양,대조조4용미부재항원태적DC단독배양.4개대조조야가입동실험조상동적각충인자진행배양.51Cr석방법검측특이성CTL적살상활성.결과 역묘유도적특이성CTL대신암세포적살상활성위(31.93±5.05)%,여대조조(5.88±2.26)%、(8.03±6.70)%、(9.70±2.09)%、(9.35±3.58)%상비차이유통계학의의(P<0.05).결론 부재항원태적DC역묘체외시험유고효적항신암세포활성.
Objective To investigate the antitumor effect of specific cytotoxic T lymphocytes induced by renal cell carcinoma multiple antigen peptide dendritic cell (DC) vaccine in vitro. Methods Dendritic cells were induced by hGM-CSF,hIL-4 from blood.Peptide of renal cell carcinoma cell line (RCC786-0)was got by citrate-phosphate buffer elution.Peripheral blood mononuclear cell was cultured.Multiple antigen peptide DC cell vaccine was obtained by acid-eluted peptide pulsed DC.The tumor antigen specific CTL was generated from activated T cell by vaccine.Killing activity of the tumor antigen specific CTL was activated by vaccine. Results Antigen peptide DC cell vaccine could show a strong cytotoxic activity of CTLs(31.93±5.05%),which was much higher than control groups(5.88±2.26%,8.03±6.70%,9.70±2.09%,9.35±3.58%). Conclusion Renal cell carcinoma antigen peptide DC vaccine could show a high antitumor effect in vitro.
