中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
2期
132-134
,共3页
细胞凋亡%HRAD17%p53 AIP1
細胞凋亡%HRAD17%p53 AIP1
세포조망%HRAD17%p53 AIP1
Cell apoptosis%HRAD17%p53 AIP1
目的 探讨HRAD17对辐射损伤诱导细胞凋亡的影响及其机制.方法 将HRAD17转染人肺癌细胞A549经60Co γ射线照射后观察细胞凋亡变化.用Western blot检测凋亡相关基因表达水平,RT-PCR检测凋亡相关基因转录水平.结果 HRAD17转染可促进γ射线诱导的细胞凋亡.与对照组相比,HRAD17转染组p53蛋白Set 46磷酸化和p53 AIP1基因转录明显增强.结论 HRAD17可促进辐射损伤诱导的细胞凋亡,其部分机制可能是通过提高p53 AIP1的表达来实现的.
目的 探討HRAD17對輻射損傷誘導細胞凋亡的影響及其機製.方法 將HRAD17轉染人肺癌細胞A549經60Co γ射線照射後觀察細胞凋亡變化.用Western blot檢測凋亡相關基因錶達水平,RT-PCR檢測凋亡相關基因轉錄水平.結果 HRAD17轉染可促進γ射線誘導的細胞凋亡.與對照組相比,HRAD17轉染組p53蛋白Set 46燐痠化和p53 AIP1基因轉錄明顯增彊.結論 HRAD17可促進輻射損傷誘導的細胞凋亡,其部分機製可能是通過提高p53 AIP1的錶達來實現的.
목적 탐토HRAD17대복사손상유도세포조망적영향급기궤제.방법 장HRAD17전염인폐암세포A549경60Co γ사선조사후관찰세포조망변화.용Western blot검측조망상관기인표체수평,RT-PCR검측조망상관기인전록수평.결과 HRAD17전염가촉진γ사선유도적세포조망.여대조조상비,HRAD17전염조p53단백Set 46린산화화p53 AIP1기인전록명현증강.결론 HRAD17가촉진복사손상유도적세포조망,기부분궤제가능시통과제고p53 AIP1적표체래실현적.
Objective To study the effects of overexpression of HRAD17 on radiation-induced apoptosis of A549 cells.Methods Transfection of FLAC-tagged HRAD17 expression plasmid into human A549 cells was carried out and flow cytometry was employed tO observe the apoptotic effect.In addition,phosphorylation of p53 Ser46 was detected by Westem blot and the transcription of p53 AIP1 was measured by RT-PCR.Results Overexpression of HRAD17 gene significantly increased radiation-induced apoptosis of A549 cells.Further.Ser46 phosphorylation of P53 protein was increased and transcription of p53 AIP1 gene was elevated in cells overexpressing HRAD17.Conclusions HRAD17 protein can sensitize A549 cells to radiation-induced apoptosis in part by increasing p53 AIP1 expression.
