CAJ | 학술논문

本文报道应用猴轮状病毒组织培养抗原制备检测轮状病毒的ELISA试剂和测定其效果的过程.应用我们的试剂和WHO轮状病毒ELISA试剂盒同时检测腹泻患者粪便标本中的轮状病毒时,阳性符合率为96.23%,阴性符合率为100.0%.应用电镜法和本试剂检测时,阳性符合率为93.33%,阴性符合率为81.82%.本试剂与含有脊髓灰质炎病毒或其他肠道细胞致病性病毒没有产生非特异性反应.结果表明我们研制的检测轮状病毒的ELISA试剂在特异性和敏感性方面是令人满意的.
본문보도응용후륜상병독조직배양항원제비검측륜상병독적ELISA시제화측정기효과적과정.응용아문적시제화WHO륜상병독ELISA시제합동시검측복사환자분편표본중적륜상병독시,양성부합솔위96.23%,음성부합솔위100.0%.응용전경법화본시제검측시,양성부합솔위93.33%,음성부합솔위81.82%.본시제여함유척수회질염병독혹기타장도세포치병성병독몰유산생비특이성반응.결과표명아문연제적검측륜상병독적ELISA시제재특이성화민감성방면시령인만의적.
This paper reports the process of utilizing the tissue culture antigen to prepare the ELISA reagent of rotavirus for the diagnosis of the infection and the determination of its efficiency.Our reagent was used and compared with the WHO ELISA reagent to detect the rotavirus in faecal specimens from the patients whith diarrhea.The positive coincidence rate (sensitivity) was 96.28% and the negative coincidence rate (specificity) was 100%.the positive coincidence rate of the results by the two methods,electronmicroscopy and ELISA,was 93.83% and the negative coincidence rate was 81.82%.There was no nonspecific reaction with faecal specimens which contained poliomyelitis virus or other enteroviruses in our test.The ELISA Kit of rotavirus will be snpplied as a commercial product.