中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
4期
337-340
,共4页
张健%李翠%秦秋%朱远凤%杨香菊%王璇%张进安
張健%李翠%秦鞦%硃遠鳳%楊香菊%王璇%張進安
장건%리취%진추%주원봉%양향국%왕선%장진안
桥本甲状腺炎%Graves病%细胞趋化因子受体-5%CD57
橋本甲狀腺炎%Graves病%細胞趨化因子受體-5%CD57
교본갑상선염%Graves병%세포추화인자수체-5%CD57
Hashimoto's thyroiditis%Graves' disease%C-X-C chemokine teceptor type 5%CD57
目的 通过观察滤泡辅助T细胞(Tfh细胞)相关因子细胞趋化因子受体-5(CXCR5)及CD57在桥本甲状腺炎及Graves病患者甲状腺的表达,探讨Tfh细胞在自身免疫性甲状腺疾病发生中的作用.方法 利用免疫组织化学SP法检测CXCR5、CD57在15例桥本甲状腺炎及18例Graves病患者甲状腺的表达,以10例正常甲状腺组织为对照组.结果 CXCR5、CD57在桥本甲状腺炎及Graves病主要表达于浸润淋巴细胞的胞膜和胞质,CXCR5阳性率分别为93.3％( 14/15)和88.9％ (16/18),CD57表达的阳性率分别为93.3％ (14/15)和83.3％ (15/18),均显著高于正常对照(P均＜0.05),两种分子在桥本甲状腺炎和Graves病中的表达差异无统计学意义(P＞0.05).结论 CXCR5及CD57主要表达在甲状腺组织浸润的淋巴细胞的胞质和胞膜,且在两种自身免疫性甲状腺疾病中的表达呈现一致性,提示Tfh细胞参与了自身免疫性甲状腺疾病的发生.
目的 通過觀察濾泡輔助T細胞(Tfh細胞)相關因子細胞趨化因子受體-5(CXCR5)及CD57在橋本甲狀腺炎及Graves病患者甲狀腺的錶達,探討Tfh細胞在自身免疫性甲狀腺疾病髮生中的作用.方法 利用免疫組織化學SP法檢測CXCR5、CD57在15例橋本甲狀腺炎及18例Graves病患者甲狀腺的錶達,以10例正常甲狀腺組織為對照組.結果 CXCR5、CD57在橋本甲狀腺炎及Graves病主要錶達于浸潤淋巴細胞的胞膜和胞質,CXCR5暘性率分彆為93.3％( 14/15)和88.9％ (16/18),CD57錶達的暘性率分彆為93.3％ (14/15)和83.3％ (15/18),均顯著高于正常對照(P均＜0.05),兩種分子在橋本甲狀腺炎和Graves病中的錶達差異無統計學意義(P＞0.05).結論 CXCR5及CD57主要錶達在甲狀腺組織浸潤的淋巴細胞的胞質和胞膜,且在兩種自身免疫性甲狀腺疾病中的錶達呈現一緻性,提示Tfh細胞參與瞭自身免疫性甲狀腺疾病的髮生.
목적 통과관찰려포보조T세포(Tfh세포)상관인자세포추화인자수체-5(CXCR5)급CD57재교본갑상선염급Graves병환자갑상선적표체,탐토Tfh세포재자신면역성갑상선질병발생중적작용.방법 이용면역조직화학SP법검측CXCR5、CD57재15례교본갑상선염급18례Graves병환자갑상선적표체,이10례정상갑상선조직위대조조.결과 CXCR5、CD57재교본갑상선염급Graves병주요표체우침윤림파세포적포막화포질,CXCR5양성솔분별위93.3％( 14/15)화88.9％ (16/18),CD57표체적양성솔분별위93.3％ (14/15)화83.3％ (15/18),균현저고우정상대조(P균＜0.05),량충분자재교본갑상선염화Graves병중적표체차이무통계학의의(P＞0.05).결론 CXCR5급CD57주요표체재갑상선조직침윤적림파세포적포질화포막,차재량충자신면역성갑상선질병중적표체정현일치성,제시Tfh세포삼여료자신면역성갑상선질병적발생.
Objective To investigate the potential role of T follicular helper cells (Tfh) in the pathogenesis of autoimmune thyroid diseases by comparing the expression of C-X-C chemokine teceptor type 5 (CXCR5) and CD57 in Hashimoto's thyroiditis (HT) and Graves' disease (GD) thyroid tissues.Methods The expression of CXCR5 and CD57 proteins was determined by immunohistochemical analysis in 15 HT thyroid samples,18 GD samples and 10 normal thyroid samples.Results Immunohistochemical staining showed that CXCR5 and CD57 were mainly positive in cytomembrane and cytoplasm of the infiltrated lymphocytes both in HT and GD tissues,with much higher levels than that of normal thyroid tissues ( P ＜ 0.05 ).Both CXCR5 and CD57 were not significantly different between the HT and GD tissues.Conclusion CXCR5 and CD57 expressions were increased with a similar expression pattern in both of the two main autoimmune thyroid diseases( AITD),indicating that Tfh may participate inthe development and progression of AITD.
