CAJ | 학술논문

目的研究单侧面肌失神经支配组织病理学改变。方法建立单侧面肌失神经支配动物模型，在失神经支配后1、3 d,1、2周,1、2、4、6个月制作面肌标本，改良Gomori三色染色观察线粒体变化；酶组化染色检测肌球蛋白ATP酶，还原型辅酶Ⅰ四唑氮还原酶（NADH-TR）；免疫组化染色检测肌球蛋白（Myosin），肌动蛋白（Actin）的表达；吖啶橙染色检测细胞内核酸含量变化。结果失神经支配初期线粒体功能加强、酶活性增高、收缩蛋白质表达增强；2周后，线粒体退化、肌纤维代谢活性降低、收缩蛋白质表达下降；1个月后肌纤维型分别开始转换；6个月组明显型组化，核酸量上升，并出现少量再生的肌纤维。结论单侧面肌失神经支配后发生典型的失神经萎缩，2～6个月有增殖改变，失神经支配1个月内是对抗肌萎缩的有利时机。
목적연구단측면기실신경지배조직병이학개변。방법건립단측면기실신경지배동물모형，재실신경지배후1、3 d,1、2주,1、2、4、6개월제작면기표본，개량Gomori삼색염색관찰선립체변화；매조화염색검측기구단백ATP매，환원형보매Ⅰ사서담환원매（NADH-TR）；면역조화염색검측기구단백（Myosin），기동단백（Actin）적표체；아정등염색검측세포내핵산함량변화。결과실신경지배초기선립체공능가강、매활성증고、수축단백질표체증강；2주후，선립체퇴화、기섬유대사활성강저、수축단백질표체하강；1개월후기섬유형분별개시전환；6개월조명현형조화，핵산량상승，병출현소량재생적기섬유。결론단측면기실신경지배후발생전형적실신경위축，2～6개월유증식개변，실신경지배1개월내시대항기위축적유리시궤。
Objective　To observe the histopathological changes in unilateral facial muscle denervation. Methods　Gomori trichrome stain, acridine orange (AO) fluorescence, enzyme-histochemistry and immunohistochemistry techniques were used for studying mitochondria, nuclei acid concentration, enzymatic activity and contraction protein expression of facial muscle in different denervation period. Results　Mitochondrian function, enzymatic activity and contraction protein expression of facial muscle denervation increased in the first two weeks, then declined till 6 months. Myofiber′s histotype began to transform in 1 month after denervation. Type-grouping was obvious in 6 month. Regeneration myofibers were also seen at this time. Conclusions　Unilateral facial muscles become atrophy after denervation. They have proliferating tendency from 2 month to 6 month. Therefore, within 1 month after denervation measures to benefit atrophy will be more effective.