广东化工
廣東化工
엄동화공
GUANGDONG CHEMICAL INDUSTRY
2012年
3期
28-29
,共2页
辣椒素%纯化%低压柱色谱%薄层色谱法
辣椒素%純化%低壓柱色譜%薄層色譜法
랄초소%순화%저압주색보%박층색보법
capsaicinoids： purification： chromatography at lower pressure%thin layer Chromatography（TLC）
从辣椒素总碱（Capsaicinoids）中提取高纯度的辣椒索及二氢辣椒素单体，建立了分离工艺条件。以TLC实验筛选适当的洗脱流动相，低压硅胶柱层析分离纯化，产物用两相法重结晶，TLC及HPLC监测分离效果及产品纯度。结果表明优选的流动相为混合溶剂石油醚：丙酮=8：2（体积比），纯化分离得到辣椒素和二氢辣椒索，纯度分别从64％和29％提高到97％和96％。
從辣椒素總堿（Capsaicinoids）中提取高純度的辣椒索及二氫辣椒素單體，建立瞭分離工藝條件。以TLC實驗篩選適噹的洗脫流動相，低壓硅膠柱層析分離純化，產物用兩相法重結晶，TLC及HPLC鑑測分離效果及產品純度。結果錶明優選的流動相為混閤溶劑石油醚：丙酮=8：2（體積比），純化分離得到辣椒素和二氫辣椒索，純度分彆從64％和29％提高到97％和96％。
종랄초소총감（Capsaicinoids）중제취고순도적랄초색급이경랄초소단체，건립료분리공예조건。이TLC실험사선괄당적세탈류동상，저압규효주층석분리순화，산물용량상법중결정，TLC급HPLC감측분리효과급산품순도。결과표명우선적류동상위혼합용제석유미：병동=8：2（체적비），순화분리득도랄초소화이경랄초색，순도분별종64％화29％제고도97％화96％。
The separation and purification of Capsaicin and Dihydrocapsaicin from Capsaicinoids was studied. The products were separated and purified with silica gel column chromatography at lower pressure, in which the suitable mobile phase was determined by thin layer chromatography. The fractions from the column chromatography cults were purified with two-phase recrystallization method and the purity was detected by HPLC. Result showed the suitable mobile phase was the mixture of ligarine and acetone with the volume ratio 8 ： 2. After column chromatography and recrystallization, two products were obtained with the purity 97 % and 96 %, which were improved from 64 % and 29 % respectively.
