防护林科技
防護林科技
방호림과기
PROTECTION FOREST SCIENCE AND TECHNOLOGY
2012年
1期
64-67
,共4页
白桦%果胶甲酯酶抑制剂%序列分析
白樺%果膠甲酯酶抑製劑%序列分析
백화%과효갑지매억제제%서렬분석
Betula platyphylla Suk.%PMEI%sequence analysis
从白桦（Betula platyphylla Suk.）cDNA文库中获得1个果胶甲酯酶抑制剂（PMEI）基因全长cDNA序列,该序列去除polyA后长946 bp,其ORF全长624 bp,编码208个氨基酸,编码蛋白的分子量22 486.4D,理论等电点为10.09,保守区分析确定其含有PMEI蛋白保守序列,属于植物PMEI超家族。该结果为研究白桦细胞壁代谢的分子调控奠定了基础。
從白樺（Betula platyphylla Suk.）cDNA文庫中穫得1箇果膠甲酯酶抑製劑（PMEI）基因全長cDNA序列,該序列去除polyA後長946 bp,其ORF全長624 bp,編碼208箇氨基痠,編碼蛋白的分子量22 486.4D,理論等電點為10.09,保守區分析確定其含有PMEI蛋白保守序列,屬于植物PMEI超傢族。該結果為研究白樺細胞壁代謝的分子調控奠定瞭基礎。
종백화（Betula platyphylla Suk.）cDNA문고중획득1개과효갑지매억제제（PMEI）기인전장cDNA서렬,해서렬거제polyA후장946 bp,기ORF전장624 bp,편마208개안기산,편마단백적분자량22 486.4D,이론등전점위10.09,보수구분석학정기함유PMEI단백보수서렬,속우식물PMEI초가족。해결과위연구백화세포벽대사적분자조공전정료기출。
A pectin methylesterase inhibitor（PMEI） gene was cloned from a cDNA library of B.platyphylla.The full cDNA length of PMEI is 946 bp when excluding the polyA tail.The open reading frame（ORF） of APX is 624 bp in length,encoding 208 amino acids;protein molecular weight is 22 486.4D,theoretical pI being 10.09.The analysis of conserved domains proves the gene contain conserved sequence of PMEI protein and belong to a member of plant PMEI superfamily.The results provide the foundation for studying molecular regulation of cell wall metabolism for B.platyphylla.
