江西科学
江西科學
강서과학
JIANGXI SCIENCE
2011年
5期
593-596,677
,共5页
原甲藻%中肋骨条藻%种间相互作用%去藻滤液
原甲藻%中肋骨條藻%種間相互作用%去藻濾液
원갑조%중륵골조조%충간상호작용%거조려액
Prorocentrom micans%Skeletonema costatum%Species interactions%Cell-free filtrates
微藻化感作用是一种极其复杂的生理、生态学现象,其在解释微藻藻种分布、群落结构及种群动力学方向起着关键作用。选择我国典型常见赤潮优势种原甲藻（Prorocentrum micans）与中肋骨条藻（Skeletonemacostatum）作为对象,研究实验室共培养条件下2种微藻种间相互作用关系。结果表明：两者以1×104 cells/mL的密度接种共培养时,原甲藻的生长状况几乎没有显著变化（F=0.004 48,P=0.947 96〉0.05）,而共培养的中肋骨条藻被原甲藻强烈抑制致死（F=5.116 63,P=0.047 21〈0.05）。Lotka-Volterra双藻竞争模型分析知,共培养条件下原甲藻的种间竞争能力大于中肋骨条藻的种间竞争能力。在去藻滤液实验中,灭菌处理及未经灭菌处理的原甲藻去藻滤液对中肋骨条藻的生长都没有显著影响（P〉0.05）;中肋骨条藻-原甲藻共培养体系的去藻滤液经灭菌处理及未经灭菌处理培养中肋骨条藻时,中肋骨条藻的生长也没有显著变化（P〉0.05）。可见,中肋骨条藻与原甲藻共培养时的种间相互作用,可以由细胞间的直接接触进行,而并不需要通过介质来完成。
微藻化感作用是一種極其複雜的生理、生態學現象,其在解釋微藻藻種分佈、群落結構及種群動力學方嚮起著關鍵作用。選擇我國典型常見赤潮優勢種原甲藻（Prorocentrum micans）與中肋骨條藻（Skeletonemacostatum）作為對象,研究實驗室共培養條件下2種微藻種間相互作用關繫。結果錶明：兩者以1×104 cells/mL的密度接種共培養時,原甲藻的生長狀況幾乎沒有顯著變化（F=0.004 48,P=0.947 96〉0.05）,而共培養的中肋骨條藻被原甲藻彊烈抑製緻死（F=5.116 63,P=0.047 21〈0.05）。Lotka-Volterra雙藻競爭模型分析知,共培養條件下原甲藻的種間競爭能力大于中肋骨條藻的種間競爭能力。在去藻濾液實驗中,滅菌處理及未經滅菌處理的原甲藻去藻濾液對中肋骨條藻的生長都沒有顯著影響（P〉0.05）;中肋骨條藻-原甲藻共培養體繫的去藻濾液經滅菌處理及未經滅菌處理培養中肋骨條藻時,中肋骨條藻的生長也沒有顯著變化（P〉0.05）。可見,中肋骨條藻與原甲藻共培養時的種間相互作用,可以由細胞間的直接接觸進行,而併不需要通過介質來完成。
미조화감작용시일충겁기복잡적생리、생태학현상,기재해석미조조충분포、군락결구급충군동역학방향기착관건작용。선택아국전형상견적조우세충원갑조（Prorocentrum micans）여중륵골조조（Skeletonemacostatum）작위대상,연구실험실공배양조건하2충미조충간상호작용관계。결과표명：량자이1×104 cells/mL적밀도접충공배양시,원갑조적생장상황궤호몰유현저변화（F=0.004 48,P=0.947 96〉0.05）,이공배양적중륵골조조피원갑조강렬억제치사（F=5.116 63,P=0.047 21〈0.05）。Lotka-Volterra쌍조경쟁모형분석지,공배양조건하원갑조적충간경쟁능력대우중륵골조조적충간경쟁능력。재거조려액실험중,멸균처리급미경멸균처리적원갑조거조려액대중륵골조조적생장도몰유현저영향（P〉0.05）;중륵골조조-원갑조공배양체계적거조려액경멸균처리급미경멸균처리배양중륵골조조시,중륵골조조적생장야몰유현저변화（P〉0.05）。가견,중륵골조조여원갑조공배양시적충간상호작용,가이유세포간적직접접촉진행,이병불수요통과개질래완성。
Algal allelopathy is a manifold ecological/physiological phenomenon which plays a crucial role in explaining microalgae distribution,community structure and the dynamics of populations within an ecosystem.In this article,interactions between Prorocentrum micans and Skeletonema costatum,causative red tide dinoflagellates,were investigated using bi-algal cultures under stable environmental conditions（temperature,light and illuminating periodicity,etc.） and sufficient nutrition supply by f/2 enrichment.The results showed that the growth of P.micans was not significantly different when cultured with S.costatum both in 1×104 cells/mL cell densities（F=0.004 48,P=0.947 960.05）,whereas the growth of S.costatum was not inhibited when cultured with P.micans in 1×104 cells/mL inoculation density（F=5.116 63,P=0.047 210.05）.Culture filtrate of P.micans induced no inhibitory effect on the growth of S.costatum,and similarly when separated by a membrane filter,S.costatum grew well when cultured in filtrate of P.micans-S.costatum,which implies that the inhibitory effect of P.micans on S.costatum in the bi-algal cultures occurred mainly by direct cell contact.The growth of P.micans and S.costatum in bi-algal cultures was simulated using a mathematical model（Lotka-Volterra） to quantify the interaction.It is indicated that P.micans outcompeted S.costatum over time.The algal interactions between P.micans and S.costatum may play an important role during the process of red tides algal species singularity and succession.
