重庆文理学院学报：自然科学版
重慶文理學院學報：自然科學版
중경문이학원학보：자연과학판
Journal of Chongqing University of Arts and Sciences
2011年
3期
46-48
,共3页
多巴胺%L-半胱氨酸%修饰电极%电化学行为
多巴胺%L-半胱氨痠%脩飾電極%電化學行為
다파알%L-반광안산%수식전겁%전화학행위
Dopamine%L-Cysteine%modified electrode%electrochemical behavior
以L-半胱氨酸作为电极修饰剂,采用循环伏安法研究L-Cys/GC电极的制备和DA在该修饰电极的电化学行为及其测定.DA在pH=6.684的磷酸盐缓冲溶液中,在L-Cys/GC电极上产生一对灵敏的氧化还原峰,峰电位分别为Epa=0.180 V和Epc=0.125 V（vs.SCE）.同时用伏安法测定DA的线性范围为1×10-3～1.0×10-6 mol/L,检出限可低达1.0×10-7mol/L（S/N=3）.对1×10-4 mol/L DA平行测定50次,其相对标准偏差约为2.5%.该电极可望进一步发展为微电极,用于生物活体内的神经递质DA的实际检测.
以L-半胱氨痠作為電極脩飾劑,採用循環伏安法研究L-Cys/GC電極的製備和DA在該脩飾電極的電化學行為及其測定.DA在pH=6.684的燐痠鹽緩遲溶液中,在L-Cys/GC電極上產生一對靈敏的氧化還原峰,峰電位分彆為Epa=0.180 V和Epc=0.125 V（vs.SCE）.同時用伏安法測定DA的線性範圍為1×10-3～1.0×10-6 mol/L,檢齣限可低達1.0×10-7mol/L（S/N=3）.對1×10-4 mol/L DA平行測定50次,其相對標準偏差約為2.5%.該電極可望進一步髮展為微電極,用于生物活體內的神經遞質DA的實際檢測.
이L-반광안산작위전겁수식제,채용순배복안법연구L-Cys/GC전겁적제비화DA재해수식전겁적전화학행위급기측정.DA재pH=6.684적린산염완충용액중,재L-Cys/GC전겁상산생일대령민적양화환원봉,봉전위분별위Epa=0.180 V화Epc=0.125 V（vs.SCE）.동시용복안법측정DA적선성범위위1×10-3～1.0×10-6 mol/L,검출한가저체1.0×10-7mol/L（S/N=3）.대1×10-4 mol/L DA평행측정50차,기상대표준편차약위2.5%.해전겁가망진일보발전위미전겁,용우생물활체내적신경체질DA적실제검측.
In this paper,the preparation of L-Cysteine modified glassy carbon electrode is successfully accomplished by cyclic voltammetry with the help of L-Cysteine as a dressing agent.The electrochemical behavior and voltammetric determination of dopamine are studied at the L-Cysteine modified electrode.In pH 6.684 phosphate buffer solution,one pair of oxidation and reduction peaks with E_pa=0.180 V and E_pc=0.125 V（vs.SCE） are obtained.At the same time,the linear range for the determination of dopamine is 1×10-3 ~ 1.0×10-6 mol/L with the detection limit of 1.0×10-7mol/L.The relative standard deviation is 2.5%（n=50,1×10-4 mol/L dopamine）.It shows that the modified electrode is expected to the development of microelectrodes for practical determination of neurotransmitter dopamine in vivo.
