CAJ | 학술논문

提取山药基因组和叶绿体DNA作为模板,分别扩增山药核糖体18S rDNA和叶绿体核糖体16S rDNA片段.测序获得1646bp的山药核糖体18S rDNA和1351bp的山药叶绿体核糖体16S rDNA,GenBank登录号分别是JF703101和JF703100.将以上两个基因片段分别与GenBank中的一些物种的相关序列进行序列对比,结果表明,18S rDNA序列相似度较大,物种间差异较小.而叶绿体16S rDNA序列在分析不同科的物种演化关系上有较高的分辨率,为分子水平上山药资源的研究提供了资料.
제취산약기인조화협록체DNA작위모판,분별확증산약핵당체18S rDNA화협록체핵당체16S rDNA편단.측서획득1646bp적산약핵당체18S rDNA화1351bp적산약협록체핵당체16S rDNA,GenBank등록호분별시JF703101화JF703100.장이상량개기인편단분별여GenBank중적일사물충적상관서렬진행서렬대비,결과표명,18S rDNA서렬상사도교대,물충간차이교소.이협록체16S rDNA서렬재분석불동과적물충연화관계상유교고적분변솔,위분자수평상산약자원적연구제공료자료.
With genome and chloroplast DNA taken as template,Dioscorea opposita 18S rDNA and chloroplast 16S rDNA fragments were amplified.A 1646bp Dioscorea opposita 18S rDNA（JF703101） and a 1351bp chloroplast 16S rDNA（JF703100） were sequenced and submitted to GenBank.Analysis of the two fragments with related sequences in GenBank shows that greater similarity lies in 18S rDNA,while chloroplast 16S rDNA has a good potential in Phylogenetic analysis of species in different family.The paper provides data for further research of Dioscorea opposita in molecular level.