CAJ | 학술논문

目的:探讨核因子-KB (nuclear factor-κB,NF-KB)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在高氧暴露早产鼠视网膜的表达规律及地塞米松对NF-KB和VEGF表达的影响.方法:标准SD大鼠的3日龄早产鼠36只随机分为3组,高氧组、干预组、空气组,其中高氧组和干预组均吸入氧气(氧浓度≥90%)7天返回空气5天,空气组一直置同一室内空气中,干预组同时每天腹腔注射地塞米松1.0 mg/ kg,高氧组和空气组每天腹腔注射等量生理盐水.用免疫组织化学法检测各组早产鼠视网膜NF-KB和VEGF表达水平,记数突破视网膜内界膜的内皮细胞核.结果:空气组NF-KB、VEGF均呈低表达,未见或罕见突破内界膜的内皮细胞核;与空气组相比,高氧组突破内界膜的内皮细胞核明显增多,NF-KB及VEGF的表达均显著增加(P分别<0.001,0.001,P<0.01);与高氧组相比,干预组突破内界膜的内皮细胞核明显减少,NF-KB及VEGF的表达均显著下降(P分别<0.05,0.001,0.05);NF-KB与VEGF,NF-KB与新生血管数及VEGF与新生血管数呈正相关( r 分别为0.47,0.56,0.45,P分别<0.05,0.01,0.05.结论:高氧暴露早产鼠视网膜内可观察到 VEGF和 NF-KB的高表达,地塞米松使高氧暴露早产鼠视网膜NF-KB和VEGF 的表达明显下降,突破视网膜内界膜的内皮细胞核数量明显减少,减少新生血管的形成,对高氧性视网膜病有一定的保护作用.
목적:탐토핵인자-KB (nuclear factor-κB,NF-KB)화혈관내피생장인자(vascular endothelial growth factor,VEGF)재고양폭로조산서시망막적표체규률급지새미송대NF-KB화VEGF표체적영향.방법:표준SD대서적3일령조산서36지수궤분위3조,고양조、간예조、공기조,기중고양조화간예조균흡입양기(양농도≥90%)7천반회공기5천,공기조일직치동일실내공기중,간예조동시매천복강주사지새미송1.0 mg/ kg,고양조화공기조매천복강주사등량생리염수.용면역조직화학법검측각조조산서시망막NF-KB화VEGF표체수평,기수돌파시망막내계막적내피세포핵.결과:공기조NF-KB、VEGF균정저표체,미견혹한견돌파내계막적내피세포핵;여공기조상비,고양조돌파내계막적내피세포핵명현증다,NF-KB급VEGF적표체균현저증가(P분별<0.001,0.001,P<0.01);여고양조상비,간예조돌파내계막적내피세포핵명현감소,NF-KB급VEGF적표체균현저하강(P분별<0.05,0.001,0.05);NF-KB여VEGF,NF-KB여신생혈관수급VEGF여신생혈관수정정상관( r 분별위0.47,0.56,0.45,P분별<0.05,0.01,0.05.결론:고양폭로조산서시망막내가관찰도 VEGF화 NF-KB적고표체,지새미송사고양폭로조산서시망막NF-KB화VEGF 적표체명현하강,돌파시망막내계막적내피세포핵수량명현감소,감소신생혈관적형성,대고양성시망막병유일정적보호작용.