CAJ | 학술논문

背景:细胞移植疗效监测取决于有效的标记方法,从而能够对移植细胞进行示踪.超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide,SPIO)是一种较为理想的新型磁共振对比剂,用SPIO标记细胞可实现对移植细胞进行活体示踪.而荧光活性染料CM-Dil无细胞毒性,不影响细胞的生长,适合标记和示踪细胞.目的:观察SPIO及CM-Dil对骨髓间充质干细胞的标记及示踪效果.方法:全骨髓法培养猪骨髓间充质干细胞,用含50 mg/L铁浓度的SPIO及CM-Dil标记骨髓间充质干细胞,将双标后的骨髓间充质干细胞经冠状动脉注入猪心肌梗死模型.4周后取心脏组织行冰冻切片观察.结果与结论:SPIO及CM-Dil在体外标记骨髓间充质干细胞效率高,几乎达100%.经冠状动脉移植4周后心肌组织可找到双标记的骨髓间充质干细胞,结果提示SPIO及CM-Dil双标记骨髓间充质干细胞体内示踪效果好.
배경:세포이식료효감측취결우유효적표기방법,종이능구대이식세포진행시종.초순자성양화철납미입자(superparamagnetic iron oxide,SPIO)시일충교위이상적신형자공진대비제,용SPIO표기세포가실현대이식세포진행활체시종.이형광활성염료CM-Dil무세포독성,불영향세포적생장,괄합표기화시종세포.목적:관찰SPIO급CM-Dil대골수간충질간세포적표기급시종효과.방법:전골수법배양저골수간충질간세포,용함50 mg/L철농도적SPIO급CM-Dil표기골수간충질간세포,장쌍표후적골수간충질간세포경관상동맥주입저심기경사모형.4주후취심장조직행빙동절편관찰.결과여결론:SPIO급CM-Dil재체외표기골수간충질간세포효솔고,궤호체100%.경관상동맥이식4주후심기조직가조도쌍표기적골수간충질간세포,결과제시SPIO급CM-Dil쌍표기골수간충질간세포체내시종효과호.
BACKGROUND:The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo.Superparamagnetic iron oxide(SPIO)labeling is an ideal magnetic resonance contrast medium,and it offers the potential for non-invasive tracking of implanted cells.The chlormethylbenzamido-1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (CM-Dil)labeling does not have cytotoxicity,so it cannot influence cell growth.OBJECTIVE:To investigate the effects of SPIO and CM-Dil labeling and tracking on bone marrow mesenchymal stem cells(BMSCs).METHODS:Porcine BMSCs were isolated and cultured by the whole bone marrow method.BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-Dil.The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion.The frozen sections of the cardiac tissues were obtained after 4 weeks.RESULTS AND CONCLUSION:Efficiency of SPIO and CM-Dil labeling BMSCs was nearly 100%.The SPIO and CM-Dil labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation.SPIO and CM-Dil labeling BMSCs were efficiently tracked in vivo.