鲤鱼挥发性成分测定及其产生机理初探
리어휘발성성분측정급기산생궤리초탐
Determination of Volatile Components in Cyprinus carpio and Study on Their Generation Mechanism
  • 万方数据
    湖北农业科学 호북농업과학

    2010年 3期 688-691 ,共4页

    杨玉平%焦春海%廖涛%熊光权%程薇%王易芬%吴文锦%李小定%乔宇%李新%耿胜荣%林若泰

    양옥평%초춘해%료도%웅광권%정미%왕역분%오문금%리소정%교우%리신%경성영%림약태

    鲤鱼%挥发性成分%土臭素%2-甲基异冰片%脂肪氧化酶 鯉魚%揮髮性成分%土臭素%2-甲基異冰片%脂肪氧化酶
    리어%휘발성성분%토취소%2-갑기이빙편%지방양화매
    cyprinus carpio%volatile component%GSM%2-MIB%lipoxidase
    试验采用頂空固相微萃取(HS-SPME)和气相色谱-质谱联用法(GC-MS)测定了东湖水体的腥味成分和东湖鲤鱼体內挥发性成分.并测定了鲤鱼体內脂肪氧化酶与不饱和脂肪酸花生四烯酸的反应产物.结果表明, 东湖水体中土臭素和2-甲基异冰片浓度范围分别为1.67～5.37 ng/L和15.26～29.68ng/L.东湖鲤鱼体内含有腥味物质2-MIB,在不同组织中的浓度范围为1.8～5.1 n/kg,腥味物质GSM未检测到.鲤鱼体內脂肪氧化酶与底物花生四烯酸反应产生的物质主要有己醛、1-辛烯-3-醇、2-辛烯醛、2-壬烯醛、2,4-癸二烯醛等,主要的反应产物和鲤鱼体內主要的挥发性成分基本吻合.本试验通过产生的主要反应产物的量来间接测定酶的活性,其中酶活最强的是鱼鳞酶,其他依次为鱼鳃酶、鱼血酶、鱼肉酶和内脏酶.
    시험채용정공고상미췌취(HS-SPME)화기상색보-질보련용법(GC-MS)측정료동호수체적성미성분화동호리어체내휘발성성분.병측정료리어체내지방양화매여불포화지방산화생사희산적반응산물.결과표명, 동호수체중토취소화2-갑기이빙편농도범위분별위1.67～5.37 ng/L화15.26～29.68ng/L.동호리어체내함유성미물질2-MIB,재불동조직중적농도범위위1.8～5.1 n/kg,성미물질GSM미검측도.리어체내지방양화매여저물화생사희산반응산생적물질주요유기철、1-신희-3-순、2-신희철、2-임희철、2,4-계이희철등,주요적반응산물화리어체내주요적휘발성성분기본문합.본시험통과산생적주요반응산물적량래간접측정매적활성,기중매활최강적시어린매,기타의차위어새매、어혈매、어육매화내장매.
    In this paper, the volatile components in carp lyrics and the off-flavor components in East Lake were determined using headspace solid phase microextraction (HS-SPME) coupled with gas chromatography mass spectrometry (GC-MS), and the main reaction products of arachidonic acid and lipoxidase were also analyzed by GC-MS. The results showed that GSM and 2-MIB in East Lake were 1.67～5.37 ng/L and 15.26～29.68 ng/L respectively. The concentrations of 2-MIB in different tissues in carp lyrics ranged from 1.8 to 5.1 ng/kg, but GSM was not detected. The main reaction products of arachidonic acid and lipoxidase included hexanal, 1-octen-ol, 2-octenal, 2-nonenal and 2,4-decadienal which were similar with those volatile components in carp lyrics. The results revealed that the highest activity enzyme which quantified indirectly by the contents of the main reaction products was from scale, and the decrease enzyme activity order were from gills, blood, muscle and visceral.
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