CAJ | 학술논문

目的我国人群乙型肝炎病毒(HBV)感染率高,HBV感染血清学标志物是临床实验室最主要的检测项目之一.本实验分析ELISA法检测HBV标志物s抗原(HBsAg)出现假阳性的原因并探讨应对措施.方法 ELISA法检测HBsAg阳性时,应用MEIA法复检.结果 156例样品用MEIA法复检HBsAgELISA法HBsAg阳性标本,结果4例阴性,ELISA法阴性误检率为2.56%.结论 ELISA法本实验室试剂存在HBsAg假阳性.在日常工作中,对ELISA法测定的HBV标志物5项指标HBsAg阳性的样品,严格多家试剂联合复检HBsAg是非常必要的.
목적 아국인군을형간염병독(HBV)감염솔고,HBV감염혈청학표지물시림상실험실최주요적검측항목지일.본실험분석ELISA법검측HBV표지물s항원(HBsAg)출현가양성적원인병탐토응대조시.방법 ELISA법검측HBsAg양성시,응용MEIA법복검.결과 156례양품용MEIA법복검HBsAgELISA법HBsAg양성표본,결과4례음성,ELISA법음성오검솔위2.56%.결론 ELISA법본실험실시제존재HBsAg가양성.재일상공작중,대ELISA법측정적HBV표지물5항지표HBsAg양성적양품,엄격다가시제연합복검HBsAg시비상필요적.
Objective Serological assay for hepatitis B virus(HBV)infection was one of important parameters in China because of high prevalence,We try to analyze the cause of false positive results of HBsAg during ELISA examination,and to find the possible solution.Methods Selected the positive specimen of HBsAg by ELISA,then examined them again by MELA.Results A-mong 156 sere positive of HBsAg reexamined by MEIA,4 specimen were negative,the false-positive rate was 2.56% for ELISA.Conclusion ELISA examination can lead to false-positive results of HBsAg,it is necessary to reexamine it with different reagents.