CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周血白细胞中干扰素诱导表达2',5'-寡腺苷酸合成酶L(OASL)和干扰素诱导蛋白2(IFIT2)基因的实时定量表达水平与SLE疾病特异性和疾病活动的相关性.方法收集50例SLE患者,25例非SLE自身免疫性疾病患者,25例健康对照人群的临床资料,取外周血抽提总RNA并逆转录成cDNA,运用SYBR green dyeI实时定量聚合酶链反应(PCR)在Bio-Rad 96孔基因测序仪上检测SLE患者组和非SLE患者组及健康对照组的OASL和IFIT2 mRNA定量表达水平(以ΔCT表示)的差异,并根据各病情不同进行分组比较,以及对其与SLEDAI和临床各相关指标相关性进行评价分析.结果 SLE患者总体OASL mRNA ΔCT值(4.83±0.41)与非SLE患者(3.26±0.47)差异有统计学意义(P=0.029),与健康对照者(3.07±0.54)差异有统计学意义(P=0.014).SLE患者总体IFIT2 mRNA ΔCT值(2.85±0.41)与非SLE患者(1.19±0.52)差异有统计学意义(P=0.019),与健康对照组(1.07±0.47)差异有统计学意义(P=0.011).SLE患者总体OASL mRNA ΔCT值与SLEDAI有相关性(r=0.324,P=0.022),与免疫球蛋白A(IgA)有相关性(r=0.357,P=0.012), IFIT2 mRNA ΔCT值与SLEDAI有相关性(r=0.399,P=0.004),与血白细胞有相关性(r=0.393,P=0.005),与IgA有相关性(r=0.283,P=0.049).结论 OASL和IFIT2在SLE患者中均表达上调,对SLE患者的病情活动度判断有意义,两者在SLE发病中均有一定作用,抑制其过度表达可能成为治疗SLE的新方法.
목적 탐토계통성홍반랑창(SLE)환자외주혈백세포중간우소유도표체2',5'-과선감산합성매L(OASL)화간우소유도단백2(IFIT2)기인적실시정량표체수평여SLE질병특이성화질병활동적상관성.방법 수집50례SLE환자,25례비SLE자신면역성질병환자,25례건강대조인군적림상자료,취외주혈추제총RNA병역전록성cDNA,운용SYBR green dyeI실시정량취합매련반응(PCR)재Bio-Rad 96공기인측서의상검측SLE환자조화비SLE환자조급건강대조조적OASL화IFIT2 mRNA정량표체수평(이ΔCT표시)적차이,병근거각병정불동진행분조비교,이급대기여SLEDAI화림상각상관지표상관성진행평개분석.결과 SLE환자총체OASL mRNA ΔCT치(4.83±0.41)여비SLE환자(3.26±0.47)차이유통계학의의(P=0.029),여건강대조자(3.07±0.54)차이유통계학의의(P=0.014).SLE환자총체IFIT2 mRNA ΔCT치(2.85±0.41)여비SLE환자(1.19±0.52)차이유통계학의의(P=0.019),여건강대조조(1.07±0.47)차이유통계학의의(P=0.011).SLE환자총체OASL mRNA ΔCT치여SLEDAI유상관성(r=0.324,P=0.022),여면역구단백A(IgA)유상관성(r=0.357,P=0.012), IFIT2 mRNA ΔCT치여SLEDAI유상관성(r=0.399,P=0.004),여혈백세포유상관성(r=0.393,P=0.005),여IgA유상관성(r=0.283,P=0.049).결론 OASL화IFIT2재SLE환자중균표체상조,대SLE환자적병정활동도판단유의의,량자재SLE발병중균유일정작용,억제기과도표체가능성위치료SLE적신방법.
Objective To investigate the expression levels of 2',5'-oligoadenylate synthetase-like (OASL) and interferon induced protein with tetratricopeptide repeats 2 (IFIT2) genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity. Methods The clinical data of 50 SLE patients, 25 non-SLE patients with rheumatic diseases, and 25 normal controls were collected. Specimens of peripheral blood were collected; total RNA was extracted and transcribed into cDNA. SYBR green dye based real-time quantitative PCR method was applied to compare the expression levels (indicated as ΔCT value) of OASL and IFIT2 in patients with SLE and those in the controls. Results The ΔCT value of OASL expression level of the SLE patients was(4.83±0.41), significantly higher than those of the non-SLE patients (3.26±0.47) and normal controls (3.07±0.54, both P<0. 05), The ΔCT value of IFIT2 expression level of the SLE patients was (2.85±0.41),significantly higher than those of the non-SLE patients (1.19±0.52) and normal controls (1.07±0.47, both P<0. 05). The ΔCT value of OASL is related with the SLEDAI scores and immunoglobulin A. (Both P<0.05). The ΔCT value of IFIT2 is related with the SLEDAI scores and IgA,white blood cell (both P<0.05). Conclusion The value of IFIT2 and OASL expression level of the SLE patients is up-regulation,the real time expression levels of OASL and IFIT2 genes are associated with SLE disease activity. To inhibit the expression of OASL and IFIT2 may become a novel therapeutic approach for SLE.