河北大学学报（自然科学版）
河北大學學報（自然科學版）
하북대학학보（자연과학판）
JOURNAL OF HEBEI UNIVERSITY(NATURAL SCIENCE EDITION)
2010年
2期
185-189
,共5页
正交实验%孢子萌发率%菌丝生长%菌丝分枝率
正交實驗%孢子萌髮率%菌絲生長%菌絲分枝率
정교실험%포자맹발솔%균사생장%균사분지솔
orthogonal experiment%the germination rate of spores%the growth of hyphal%the rate of hyphal branching
为了确定AM真菌孢子萌发和菌丝生长的最佳水平条件,采用L_(16)(4~5)多因子正交实验对影响网状球囊霉孢子萌发和菌丝生长的因素进行了优化选择.结果表明:优组合为A_4B2C_2D_3E_3,即pH 8,水琼脂质量浓度10 g/L,培养周期10 d,后熟条件20 d,培养温度28 ℃为最佳组合.同时发现在优组合下孢子不仅萌发得早,萌发率高,菌丝生长也很旺盛.
為瞭確定AM真菌孢子萌髮和菌絲生長的最佳水平條件,採用L_(16)(4~5)多因子正交實驗對影響網狀毬囊黴孢子萌髮和菌絲生長的因素進行瞭優化選擇.結果錶明:優組閤為A_4B2C_2D_3E_3,即pH 8,水瓊脂質量濃度10 g/L,培養週期10 d,後熟條件20 d,培養溫度28 ℃為最佳組閤.同時髮現在優組閤下孢子不僅萌髮得早,萌髮率高,菌絲生長也很旺盛.
위료학정AM진균포자맹발화균사생장적최가수평조건,채용L_(16)(4~5)다인자정교실험대영향망상구낭매포자맹발화균사생장적인소진행료우화선택.결과표명:우조합위A_4B2C_2D_3E_3,즉pH 8,수경지질량농도10 g/L,배양주기10 d,후숙조건20 d,배양온도28 ℃위최가조합.동시발현재우조합하포자불부맹발득조,맹발솔고,균사생장야흔왕성.
In order to determine the optimal level condition of spore germination and hyphal growth, several factors that influence spores germination and hyphal growth of arbuscular mycorrhizal fungi (AMF) were optimized by L_(16)(4~5) multifactor orthogonal experiment and the best level of spore germination was determined. The results showed that the best technical conditions were as following: pH 8, 10 g/L of water agar, 10 days of culture cycle, 20 days of aging storage, 28 ℃ of culture temperature. We found not only the spore germination was earlier and the germination rate of spores was higher, but also the hyphal growth was more stronger under the optimal condition.
