CAJ | 학술논문

本研究采用AFLP和SRAP分子标记技术对9个哺鸡竹类竹种(含2个变型)的亲缘关系进行分析.研究结果表明:12对AFLP引物共扩增出359条带,多态性条带比率为49.7%,相似系数变化范围在0.779～0.978之间;24对SRAP引物共扩增出258条带,多态性条带比率为64.O%,相似系数变化范围在0.721～0.958之间.UPGMA聚类分析表明,AFLP和SRAP标记均将哺鸡竹分成2大类:第Ⅰ类为富阳乌哺鸡竹、花哺鸡竹、黄纹竹、黄秆乌哺鸡竹、毛壳花哺鸡竹、乌哺鸡竹、白哺鸡竹;第Ⅱ类为云和乌哺鸡竹、红哺鸡竹.对这两种标记相似性系数进行相关性分析,AFLP与SRAP的结果呈极显著相关(r=0.934),但SRAP标记比AFLP标记可在竹类及其种下等级检测到更大的遗传变异.
본연구채용AFLP화SRAP분자표기기술대9개포계죽류죽충(함2개변형)적친연관계진행분석.연구결과표명:12대AFLP인물공확증출359조대,다태성조대비솔위49.7%,상사계수변화범위재0.779～0.978지간;24대SRAP인물공확증출258조대,다태성조대비솔위64.O%,상사계수변화범위재0.721～0.958지간.UPGMA취류분석표명,AFLP화SRAP표기균장포계죽분성2대류:제Ⅰ류위부양오포계죽、화포계죽、황문죽、황간오포계죽、모각화포계죽、오포계죽、백포계죽;제Ⅱ류위운화오포계죽、홍포계죽.대저량충표기상사성계수진행상관성분석,AFLP여SRAP적결과정겁현저상관(r=0.934),단SRAP표기비AFLP표기가재죽류급기충하등급검측도경대적유전변이.
In this research,amplified fragment length polymorphisms(AFLP)and sequence-related amplified polymorphisms(SRAP)molecular markers were used to detect the genetic relationship among nine Puji-bamboo species,in which there were 2 cultivars,Ph.vivax f.huangwenzhu and Ph.vivax f.aureocaulis.359 bands were generated by 12 AFLP primer combinations with polymorphic bands accounting for 49.7%and similarity coefficient ranging from 0.779 to 0.978,and 258 bands were produced by 24 SRAP primer combinations with polymorphic bands accounting for 64.0%and similarity coefficient within 0.721 to 0.958.UPGMA cluster analysis indicated that the germplasms were both divided into two clusters base on AFLP and SRAP markers,cluster Ⅰ was composed of Ph.nigella,Ph.glabrata,Ph.vivax f.huangwenzhu,Ph.vivax f.aureocaulis,Ph.circumpilis,Ph.dulcis and Ph.vivax f.vivax;Cluster Ⅱ only including Ph.yunhoensis and Ph.iridescens.Correlation analysis of similarity index between the two markers showed that the significant correlation between AFLP and SRAP markers was observed (r=0.934),and SRAP was more suitable for the analysis of genetic diversity in bamboo species and infra-species.