CAJ | 학술논문

从土壤中筛选分离到1株γ聚谷氨酸的生产菌株yt102,初步鉴定为枯草芽孢杆菌;以此为出发菌株采用紫外线(UV)、亚硝基胍(NTG)进行复合诱变,获得1株γ聚谷氨酸高产突变株,突变株连续传代10次,发酵性能稳定;通过单因素和正交试验确定培养基的最佳组成,在最优条件下,γ聚谷氨酸的平均产量可达28.5 g/L.
종토양중사선분리도1주γ취곡안산적생산균주yt102,초보감정위고초아포간균;이차위출발균주채용자외선(UV)、아초기고(NTG)진행복합유변,획득1주γ취곡안산고산돌변주,돌변주련속전대10차,발효성능은정;통과단인소화정교시험학정배양기적최가조성,재최우조건하,γ취곡안산적평균산량가체28.5 g/L.
γ-Polyglutamic acid producing strain yt102 was isolated from soils and taxonomically identified as Bacillus subtilis. A high productive strain of γ-polyglutamic acid was obtained from yt102 with mutagenesis by UV and NTG, and it was genetic ally stable in 10 generations. Fermentation medium composition for the mutant strain was optimized by a single factor and preliminary orthogonal experiments. The average yield of γ-polyglutamic acid reached 28.5 g/L on the optimized medium.