上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2010年
1期
1-4
,共4页
周熙荣%庄静%孙超才%王伟荣%李延莉%杨立勇%蒋美艳%顾龙弟%钱小芳
週熙榮%莊靜%孫超纔%王偉榮%李延莉%楊立勇%蔣美豔%顧龍弟%錢小芳
주희영%장정%손초재%왕위영%리연리%양립용%장미염%고룡제%전소방
甘蓝型油菜%显性核不育%纯合两型系%恢复系
甘藍型油菜%顯性覈不育%純閤兩型繫%恢複繫
감람형유채%현성핵불육%순합량형계%회복계
Brassica napus%Dominant genic male sterility%Homozygous two-type sterile line%Restoring line
以甘蓝型油菜双低品种"沪油15"为轮回亲本,分别以显性核不育性的Ms不育基因和Rf恢复基因为目标基因,通过杂交和回交,将目标基因导入双低品种"沪油15".通过复交将Ms不育基因和Rf恢复基因融入同一群体,然后采用自交、兄妹交和测交等育种手段,获得育性分离比例为3:1的自交后代,其基因型为1 MsMsrfrf:2 MsMsRfrf:1 MsMsRfRf,最后从该群体配置兄妹交组合,育性分离比例符合1:1的兄妹交后代就是经回交转育的双低纯合两型系"HY15AB",其基因型分别为MsMsRfrf和MsMsrfrf.
以甘藍型油菜雙低品種"滬油15"為輪迴親本,分彆以顯性覈不育性的Ms不育基因和Rf恢複基因為目標基因,通過雜交和迴交,將目標基因導入雙低品種"滬油15".通過複交將Ms不育基因和Rf恢複基因融入同一群體,然後採用自交、兄妹交和測交等育種手段,穫得育性分離比例為3:1的自交後代,其基因型為1 MsMsrfrf:2 MsMsRfrf:1 MsMsRfRf,最後從該群體配置兄妹交組閤,育性分離比例符閤1:1的兄妹交後代就是經迴交轉育的雙低純閤兩型繫"HY15AB",其基因型分彆為MsMsRfrf和MsMsrfrf.
이감람형유채쌍저품충"호유15"위륜회친본,분별이현성핵불육성적Ms불육기인화Rf회복기인위목표기인,통과잡교화회교,장목표기인도입쌍저품충"호유15".통과복교장Ms불육기인화Rf회복기인융입동일군체,연후채용자교、형매교화측교등육충수단,획득육성분리비례위3:1적자교후대,기기인형위1 MsMsrfrf:2 MsMsRfrf:1 MsMsRfRf,최후종해군체배치형매교조합,육성분리비례부합1:1적형매교후대취시경회교전육적쌍저순합량형계"HY15AB",기기인형분별위MsMsRfrf화MsMsrfrf.
Two target genes,a dominant genic male sterility gene(Ms)and a restoring gene(Rf),were transferred respectively to the double-low-content rapeseed cultivar "Huyou 15" by crossing and backcrossing,and then the two lines were crossed so as to blend Ms gene with Rf gene.By means of selfing,sib mating and test cross,the selfed progeny with the genotype of 1 MsMsrfrf:2 MsMsRfrf:1 MsMsRfRf was obtained,whose fertility segregation ratio was 3:1.At last by sib cross combination a homozygous two-type sterile line "HY15AB" including MsMsRfrf and MsMsrfrf two genotypes was obtained,and its sib cross always showed a fertility segregation ratio of 1:1.
