南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2009年
6期
35-38
,共4页
王滑%阎亚波%张俊佩%裴东
王滑%閻亞波%張俊珮%裴東
왕활%염아파%장준패%배동
核桃%铁核桃%ITS%SSR%亲缘关系
覈桃%鐵覈桃%ITS%SSR%親緣關繫
핵도%철핵도%ITS%SSR%친연관계
Juglans regia%J. sigillata%ITS%SSR%relationship
核桃与铁核桃是我国核桃属两个主要栽培种,而后者又是我国特有种,关于两个种在植物分类学上的归属问题一直存在争议.该研究分别以核桃9铁核桃的5个居群为试材,采用ITS序列多态性分析及SSR分子标记技术,从分子水平上讨论了两个种的亲缘关系.结果显示两个种的ITS序列仅出现了一个碱基差异,序列高度相似.SSR标记共扩出了65个等位基因,其中三家村居群与其宗居群的遗传距离最近,为0.119,遗传距离最远的为MN居群与HS居群,为0.522.种间居群遗传距离与种内遗传距离相近,种问差异不明显.这一结果说明两个种应为不同的地理生态型.
覈桃與鐵覈桃是我國覈桃屬兩箇主要栽培種,而後者又是我國特有種,關于兩箇種在植物分類學上的歸屬問題一直存在爭議.該研究分彆以覈桃9鐵覈桃的5箇居群為試材,採用ITS序列多態性分析及SSR分子標記技術,從分子水平上討論瞭兩箇種的親緣關繫.結果顯示兩箇種的ITS序列僅齣現瞭一箇堿基差異,序列高度相似.SSR標記共擴齣瞭65箇等位基因,其中三傢村居群與其宗居群的遺傳距離最近,為0.119,遺傳距離最遠的為MN居群與HS居群,為0.522.種間居群遺傳距離與種內遺傳距離相近,種問差異不明顯.這一結果說明兩箇種應為不同的地理生態型.
핵도여철핵도시아국핵도속량개주요재배충,이후자우시아국특유충,관우량개충재식물분류학상적귀속문제일직존재쟁의.해연구분별이핵도9철핵도적5개거군위시재,채용ITS서렬다태성분석급SSR분자표기기술,종분자수평상토론료량개충적친연관계.결과현시량개충적ITS서렬부출현료일개감기차이,서렬고도상사.SSR표기공확출료65개등위기인,기중삼가촌거군여기종거군적유전거리최근,위0.119,유전거리최원적위MN거군여HS거군,위0.522.충간거군유전거리여충내유전거리상근,충문차이불명현.저일결과설명량개충응위불동적지리생태형.
Juglans regia L. and J. sigillata Dode. are two mainly-cultivated walnut species in China, and the later is a u-nique species distributed in the southwest China. Furthermore, the argument about relationship of the two species remains undetermined. Five populations of J. regia and J. sigillata were chosen to investigate their relationship with ITS and SSR markers. Result of ITS sequence only detected 1 nucleotide difference between the two species. Based on 65 alleles generated with SSR, genetic distance between populations was calculated. The closest genetic distance was 0.119 found between SJC and QZ population, and the furthest distance was 0. 552 found between MN and HS population. The genetic distance between intra-species was similar to that between inter-species, and the difference between two species was quite small and was not significant. Those results suggested that the two species might be different eco-types, which provided a scientific reference for revealing the evolutional and genetic relationship and genetic characteristics of walnut plants.