CAJ | 학술논문

OsGSTLI是位于水稻3号染色体上的一个λ类谷胱甘肽转移酶基因,由8个内含子和9个外显子组成,编码一个由243个氨基酸组成的多肽链.将OsGSTLI克隆到酵母表达载体pYTV上,转化大肠杆菌,然后冉转化酵母菌PEP4.Western印迹分析表明外源OsGSTL/基因在转基因酵母中表达,分析半乳糖诱导表达的酵母粗提液的谷胱甘肽转移酶活性表明:转基因酵母的谷胱甘肽转移酶较非转基因酵母和末诱导的酵母高,说明OsGSTLI在转基因酵母中受半乳糖的诱导表达,具有谷胱甘肽转移酶活性.
OsGSTLI시위우수도3호염색체상적일개λ류곡광감태전이매기인,유8개내함자화9개외현자조성,편마일개유243개안기산조성적다태련.장OsGSTLI극륭도효모표체재체pYTV상,전화대장간균,연후염전화효모균PEP4.Western인적분석표명외원OsGSTL/기인재전기인효모중표체,분석반유당유도표체적효모조제액적곡광감태전이매활성표명:전기인효모적곡광감태전이매교비전기인효모화말유도적효모고,설명OsGSTLI재전기인효모중수반유당적유도표체,구유곡광감태전이매활성.
OsGSTLI is a glutathione S-trangferase(GSTs,EC 2.5.1.18),lambda class gene in chromosome 3 of rice (Oryza sativa L.).It includes 8 introns and 9 exons,and encodes a protein of 243 amino acid residues.The OsGSTLI gene was cloned into pYTV vector and Was transformed into yeast strain PEP4.Western blot analysis showed the exogenous OsGSTLI was expressed in transformed yeast.GST activity of crude extracts of yeast showed the OsGSTLI transgenic yeast had higher levels of GST activities than control yeasts,which demonstrate the OsGSTLI has glutathione S-transferase activity.