神经科学通报（英文版）
神經科學通報（英文版）
신경과학통보（영문판）
NEUROSCIENCE BULLETIN
2008年
6期
351-358
,共8页
韩宁%丁素菊%吴涛%朱幼丽
韓寧%丁素菊%吳濤%硃幼麗
한저%정소국%오도%주유려
脑出血%自由基%凋亡%末端转移酶标记技术%依达拉奉
腦齣血%自由基%凋亡%末耑轉移酶標記技術%依達拉奉
뇌출혈%자유기%조망%말단전이매표기기술%의체랍봉
intracerebral hemorrhage%free radical%apoptosis%TUNEL%edaravone
目的 研究脑出血后血肿周围组织不同自由基水平对于细胞凋亡的影响.方法 成年SD大鼠随机分为4组:假手术组、模型组、1mg/kg依达拉奉组、3 mg/kg依达拉奉组,各组又根据造模后处死动物的不同时间(6 h,12 h,24 h,48 h,72 h,7 d,14 d)分为七个亚组,假手术组中每个亚组1只大鼠,其余三组中每个亚组6只大鼠.左尾壳核立体定向注入自体血80 μL,制作大鼠脑出血动物模型.分光光度计法检测血肿周围丙二醛(mflonaldehyde,MDA)及羟自由基含量,末端转移酶标记技术(terminal deoxynucleotidyltransfemse-mediated dUTP-biotin nick end labeling,TLINEL)检测血肿组织周围细胞凋亡数,并分析血肿周围组织自由基水平和凋亡相关性.结果 (1)模型组羟自由基及MDA含量较假手术组明显增加,四组间进行统计学分析,具有显著性差异.(2)模型组和两种剂量的依达拉奉组均于6 h即可观察到TUNEL阳性细胞,24 h明显增加,72 h时达到高峰,7 d时明显减少,14 d时于血肿周边仍可见少量阳性细胞.(3)凋亡细胞数与脑组织产生自由基能力(r=0.2003)及MDA含量(r=0.6563)具有相关性.结论 脑出血后细胞凋亡数和自由基水平变化趋势相同,二者具有相关性,提示自由基可能参与诱导出血后神经元及胶质细胞凋亡.
目的 研究腦齣血後血腫週圍組織不同自由基水平對于細胞凋亡的影響.方法 成年SD大鼠隨機分為4組:假手術組、模型組、1mg/kg依達拉奉組、3 mg/kg依達拉奉組,各組又根據造模後處死動物的不同時間(6 h,12 h,24 h,48 h,72 h,7 d,14 d)分為七箇亞組,假手術組中每箇亞組1隻大鼠,其餘三組中每箇亞組6隻大鼠.左尾殼覈立體定嚮註入自體血80 μL,製作大鼠腦齣血動物模型.分光光度計法檢測血腫週圍丙二醛(mflonaldehyde,MDA)及羥自由基含量,末耑轉移酶標記技術(terminal deoxynucleotidyltransfemse-mediated dUTP-biotin nick end labeling,TLINEL)檢測血腫組織週圍細胞凋亡數,併分析血腫週圍組織自由基水平和凋亡相關性.結果 (1)模型組羥自由基及MDA含量較假手術組明顯增加,四組間進行統計學分析,具有顯著性差異.(2)模型組和兩種劑量的依達拉奉組均于6 h即可觀察到TUNEL暘性細胞,24 h明顯增加,72 h時達到高峰,7 d時明顯減少,14 d時于血腫週邊仍可見少量暘性細胞.(3)凋亡細胞數與腦組織產生自由基能力(r=0.2003)及MDA含量(r=0.6563)具有相關性.結論 腦齣血後細胞凋亡數和自由基水平變化趨勢相同,二者具有相關性,提示自由基可能參與誘導齣血後神經元及膠質細胞凋亡.
목적 연구뇌출혈후혈종주위조직불동자유기수평대우세포조망적영향.방법 성년SD대서수궤분위4조:가수술조、모형조、1mg/kg의체랍봉조、3 mg/kg의체랍봉조,각조우근거조모후처사동물적불동시간(6 h,12 h,24 h,48 h,72 h,7 d,14 d)분위칠개아조,가수술조중매개아조1지대서,기여삼조중매개아조6지대서.좌미각핵입체정향주입자체혈80 μL,제작대서뇌출혈동물모형.분광광도계법검측혈종주위병이철(mflonaldehyde,MDA)급간자유기함량,말단전이매표기기술(terminal deoxynucleotidyltransfemse-mediated dUTP-biotin nick end labeling,TLINEL)검측혈종조직주위세포조망수,병분석혈종주위조직자유기수평화조망상관성.결과 (1)모형조간자유기급MDA함량교가수술조명현증가,사조간진행통계학분석,구유현저성차이.(2)모형조화량충제량적의체랍봉조균우6 h즉가관찰도TUNEL양성세포,24 h명현증가,72 h시체도고봉,7 d시명현감소,14 d시우혈종주변잉가견소량양성세포.(3)조망세포수여뇌조직산생자유기능력(r=0.2003)급MDA함량(r=0.6563)구유상관성.결론 뇌출혈후세포조망수화자유기수평변화추세상동,이자구유상관성,제시자유기가능삼여유도출혈후신경원급효질세포조망.
Objective To investigate the correlation of perihematomal free radical level and neuronal apoptosis following the intracerebral hemorrhage (ICH). Methods Animals were randomly divided into 4 groups: sham operation group, model group, 1 mg/kg edaravone group, and 3 mg/kg edaravone group. Each group was then divided into seven subgroups, in which the rats were correspondingly killed at 6 h, 12 h, 24 h, 48 h, 72 h, 7 d or 14 d (n = 1 in each subgroup of the sham group, and n = 6 in each subgroup of the other 3 groups). By Horseley-Clarke technique, autoblood (80 μL) were administered into the left caudate putamen of SD rats in a double administration-withdrawal way. Rats in the sham group were needled in but not administered with autoblood. The ICH model was then evaluated by Bederson's scale. Around the hematoma, the levels of malonaldehyde (MDA) and hydroxyl radical were tested by spectrophotometer, and the process of apoptosis was tested by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) method. Results (1) ICH significantly increased the levels of MDA and hydroxyl radicals. Significant differences in MDA and hydroxyl radical contents were observed among the four groups. (2) In the sham group, a small number of TUNEL-positive cells were found. In the other three groups, the TUNEL-positive cells were observed at 6 h, increased significantly at 24 h, and reached peak level at 3 d, then fell profoundly at 7 d, but remained detectable at 14 d. (3) The positive correlation existed between apoptosis and free radical level (r = 0.2003), and existed between apoptosis and MDA content (r= 0.6563) in the brain. Conclusion Post-hemorrhagic apoptosis was related to the production of free radicals, indicating that the elevated free radicals following the ICH could induce neuron and glial cell apoptosis.
