中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2008年
1期
38-39
,共2页
侍晓云%畅继武%邱明才%隋志芳%马富玲
侍曉雲%暢繼武%邱明纔%隋誌芳%馬富玲
시효운%창계무%구명재%수지방%마부령
胰岛素基因%HepG-2细胞%表达调控%葡萄糖%胰岛素
胰島素基因%HepG-2細胞%錶達調控%葡萄糖%胰島素
이도소기인%HepG-2세포%표체조공%포도당%이도소
Insulin
用脂质体介导法将质粒p(GIRE)3BP-11×Furin转染至HepG-2细胞.培养该细胞,以不同浓度的葡萄糖或胰岛素加入培养基.结果 显示,葡萄糖和胰岛素均能浓度依赖地分别刺激和抑制胰岛素基因的表达.
用脂質體介導法將質粒p(GIRE)3BP-11×Furin轉染至HepG-2細胞.培養該細胞,以不同濃度的葡萄糖或胰島素加入培養基.結果 顯示,葡萄糖和胰島素均能濃度依賴地分彆刺激和抑製胰島素基因的錶達.
용지질체개도법장질립p(GIRE)3BP-11×Furin전염지HepG-2세포.배양해세포,이불동농도적포도당혹이도소가입배양기.결과 현시,포도당화이도소균능농도의뢰지분별자격화억제이도소기인적표체.
The plasmid p(GIRE)3BP-11× Furin was transfected into HepG-2 cells by liposome-mediated method and the cells were cultured in different concentrations of glucose and insulin.The insulin mRNA expressions were stimulated by glucose and inhibited by insulin in a concentration-dependent manner.
