CAJ | 학술논문

目的探讨E-选择素在胰岛素抵抗(IR)大鼠血管内皮的表达及其与相关影响因素的关系.方法 Wistar大鼠随机分为3组:高脂膳食组(HF组),高糖膳食组(HG组)与对照组(NC组).高脂或高糖膳食诱导IR大鼠模型,正葡萄糖钳夹技术验证模型制备成功.Western印记法检测E-选择素蛋白表达.结果 HF组与HG组E-选择素的表达高于NC组(P＜0.01),HF组高于HG组(P＜0.05);在HF组和HG组,E-选择素的表达与TG、FFA、FIns、HOMA-IR呈正相关(P＜0.05),且与HOMA-IR相关性最强(HF组P=0.01,HG组P=0.009).结论 IR可能是E-选择素表达增加的原因之一.
목적 탐토E-선택소재이도소저항(IR)대서혈관내피적표체급기여상관영향인소적관계.방법 Wistar대서수궤분위3조:고지선식조(HF조),고당선식조(HG조)여대조조(NC조).고지혹고당선식유도IR대서모형,정포도당겸협기술험증모형제비성공.Western인기법검측E-선택소단백표체.결과 HF조여HG조E-선택소적표체고우NC조(P＜0.01),HF조고우HG조(P＜0.05);재HF조화HG조,E-선택소적표체여TG、FFA、FIns、HOMA-IR정정상관(P＜0.05),차여HOMA-IR상관성최강(HF조P=0.01,HG조P=0.009).결론 IR가능시E-선택소표체증가적원인지일.
Objective To discuss the expression and the related factors of E-selectin in insulin-resistant(IR)rat endothelium. Methods Wistar rats were randomly divided into 3 groups: high fat feed,high fructose feed and the control feed(HF group,HG group and NC group). IR rat model was established by high fat feeding or high fructose feeding for 4 weeks and was evaluated with euglycemic clamp technique. E-selectin protein expression was detected by Western-blot analysis. Results The expression of E-selectin protein in vessel endothelium was increased in HF group and HG group compared with that in NC group (P<0.01), and higher in HF group than in HG group (P<0.05). E-selectin protein expression was correlated significantly with TG, FFA, FIns and HOMA-IR in HF group and HG group (P<0.05). Conclusions IR may cause the increased expression of E-selectin in endothelium of rats fed with high fat or fructose.