CAJ | 학술논문

本工作观察了自发性高血压大鼠(SHRs)和Wistar-kyoto (WKY)大鼠心肌和主动脉肾上腺髓质素(adrenomedullin, ADM)和肾上腺髓质素原N-末端20肽(proadrenomedullin N terminal 20 peptide, PAMP)的水平.以放射免疫分析方法测定血浆、心肌和主动脉ADM含量.用竞争性定量逆转录多聚酶链式反应(RT-PCR)方法测定心肌和主动脉ProADM mRNA含量.结果发现, SHRs心肌和主动脉ProADM mRNA水平分别比WKY大鼠高66.7% 和73%(均P<0.01).SHRs血浆、心肌和主动脉ADM-ir含量分别较WKY大鼠高29%、76.7%和79%(均P<0.01).SHRs血浆、心肌和主动脉PAMP-ir水平分别较WKY大鼠高42.5%(P<0.01)、47.2%(P<0.01)和27.3%(P<0.05).另外, SHRs 的ADM 和PAMP的比值较WKY大鼠明显增高(心肌和主动脉分别为2.0±0.25 vs 1.64±0.3和 2.2±0.18 vs 1.56±0.28).结果提示, SHRs心肌和主动脉ProADM基因表达上调, ADM和PAMP水平升高, 但二者升高的比例不一致.SHRs 的ADM和PAMP升高不一致的病理生理意义有待进一步研究.
본공작관찰료자발성고혈압대서(SHRs)화Wistar-kyoto (WKY)대서심기화주동맥신상선수질소(adrenomedullin, ADM)화신상선수질소원N-말단20태(proadrenomedullin N terminal 20 peptide, PAMP)적수평.이방사면역분석방법측정혈장、심기화주동맥ADM함량.용경쟁성정량역전록다취매련식반응(RT-PCR)방법측정심기화주동맥ProADM mRNA함량.결과발현, SHRs심기화주동맥ProADM mRNA수평분별비WKY대서고66.7% 화73%(균P<0.01).SHRs혈장、심기화주동맥ADM-ir함량분별교WKY대서고29%、76.7%화79%(균P<0.01).SHRs혈장、심기화주동맥PAMP-ir수평분별교WKY대서고42.5%(P<0.01)、47.2%(P<0.01)화27.3%(P<0.05).령외, SHRs 적ADM 화PAMP적비치교WKY대서명현증고(심기화주동맥분별위2.0±0.25 vs 1.64±0.3화 2.2±0.18 vs 1.56±0.28).결과제시, SHRs심기화주동맥ProADM기인표체상조, ADM화PAMP수평승고, 단이자승고적비례불일치.SHRs 적ADM화PAMP승고불일치적병리생리의의유대진일보연구.
In this study, we observed the levels of adrenomedullin (ADM) and proadrenomedullin N-terminal 20 peptide (PAMP) in myocardium and aorta of spontaneously hypertensive rats (SHRs) in comparison with Wistar-kyoto (WKY) rats. Contents of ADM and PAMP were measured by radioimmunoassay (RIA) in plasma, myocardium and aorta. The amount of Pro-ADM mRNA of myocardium and aorta was determined by competitive quantitative reverse transcription polymerase chain reaction (RT-PCR). In SHRs the amounts of Pro-ADM mRNA of myocardium and aorta were 66.7% (P<0.01) and 73% (P<0.01) higher than those in WKY rat, respectively. In SHRs, the levels of ADM in plasma, myocardium and aorta were 29%, 76.7% and 79% (all P<0.01) higher than those in WKY rats, respectively. The level of PAMP in SHRs was increased by 42.5% in plasma (P<0.01), 47.2% in myocardium (P<0.0.1) and 27.3% in aorta (P<0.05) compared to WKY rats, respectively. In addition, the ratio of ADM content to PAMP content in SHRs group was increased compared with that in WKY group (2.0±0.25 vs 1.64±0.3 and 2.2±0.18 vs 1.56±0.28, in myocardium and aorta, respectively, P<0.01). These results suggest that ProADM gene expression is up-regulated and the increase in ADM and PAMP is different in SHRs. The significance of inconsistency of increase in ADM and PAMP in SHRs needs to be further investigated.