CAJ | 학술논문

目的:筛选胰岛素受体高亲和肽作为肝细胞癌潜在靶向载体.方法:用噬菌体随机环七肽库,与鼠肝胰岛素受体亲和、筛选与扩增.经过三个循环的筛选,测定阳性噬菌体与胰岛素受体的亲和力,测定亲和力高的阳性噬菌体克隆插入DNA序列,推断出与噬菌体外壳gⅢ蛋白融合的短肽氨基酸残基序列.分析亲和短肽结构特征,固相法选择性人工合成多肽配基TK1,测定多肽TK1与胰岛素受体的亲和力.结果:经过三轮筛选,选择12个阳性克隆测序,11个克隆插入短肽是TK(Cys Gln Ser Lys His Trp Arg His Cys),合成肽TK1(Cys Gln Ser Lys His Trp Arg His Cys Tyr)与胰岛素受体有较高亲和力,Kd值为4.31×108L/M.结论:噬菌体肽库筛选小鼠肝胰岛素受体获得了两种多肽配基,多肽TK1与胰岛素受体有较高亲和力,可望作为肝癌靶向多肽,值得进一步研究.
목적:사선이도소수체고친화태작위간세포암잠재파향재체.방법:용서균체수궤배칠태고,여서간이도소수체친화、사선여확증.경과삼개순배적사선,측정양성서균체여이도소수체적친화력,측정친화력고적양성서균체극륭삽입DNA서렬,추단출여서균체외각gⅢ단백융합적단태안기산잔기서렬.분석친화단태결구특정,고상법선택성인공합성다태배기TK1,측정다태TK1여이도소수체적친화력.결과:경과삼륜사선,선택12개양성극륭측서,11개극륭삽입단태시TK(Cys Gln Ser Lys His Trp Arg His Cys),합성태TK1(Cys Gln Ser Lys His Trp Arg His Cys Tyr)여이도소수체유교고친화력,Kd치위4.31×108L/M.결론:서균체태고사선소서간이도소수체획득료량충다태배기,다태TK1여이도소수체유교고친화력,가망작위간암파향다태,치득진일보연구.