CAJ | 학술논문

目的研究骨软骨瘤和骨恶性肿瘤组织中cyclinD1和CDK4表达及其临床病理意义.方法骨肿瘤组织脱钙后常规石蜡包埋切片ABC免疫组化法.结果15例骨软骨瘤cyclinD1和CDK4表达均阴性,45例骨恶性肿瘤cyclinD1和CK4表达阳性率分别为44%(20/45)和60%(27/45).组织学分级1级和无原发灶外转移骨恶性肿瘤cyclinD1和CDK4阳性率明显低于组织学分级Ⅲ级和原发灶外转移病例,其中分级之间均存在显著差异(P＜0.05);骨恶性肿瘤中cyclinD1和CDK4表达存在密切正相关(P＜0.05).结论cyclinD1和CDK4表达与骨恶性肿瘤生物学行为和预后存在较密切关系,骨恶性肿瘤的分子发病机制可能涉及到CDK4/.cyclinD1调节通路异常,检测骨良性病变cyclinD1和CDK4的表达可能对预防和早期发现骨恶性肿瘤有一定临床意义.
목적연구골연골류화골악성종류조직중cyclinD1화CDK4표체급기림상병리의의.방법골종류조직탈개후상규석사포매절편ABC면역조화법.결과15례골연골류cyclinD1화CDK4표체균음성,45례골악성종류cyclinD1화CK4표체양성솔분별위44%(20/45)화60%(27/45).조직학분급1급화무원발조외전이골악성종류cyclinD1화CDK4양성솔명현저우조직학분급Ⅲ급화원발조외전이병례,기중분급지간균존재현저차이(P＜0.05);골악성종류중cyclinD1화CDK4표체존재밀절정상관(P＜0.05).결론cyclinD1화CDK4표체여골악성종류생물학행위화예후존재교밀절관계,골악성종류적분자발병궤제가능섭급도CDK4/.cyclinD1조절통로이상,검측골량성병변cyclinD1화CDK4적표체가능대예방화조기발현골악성종류유일정림상의의.
Objective:To detect the expressions of cyclinD1,cyclin-dependent kinase 4(CDK4)in osteochondroma and malignant bone tumors and study their clinicopathological significance.Methods:The expression of cyclinD1 and CDK4 was examined using immunohistochenmical method of avidin-biotin complex on the routinely paraffin-embedded sections after decalcification of bone tissues.Results:The expression of cyclinD1and CDK4 was all negative in 15 cases with osteochondroma,but the positive rates of cyclinDl and CDK4 in malignant bone tumors were 44%(20/45)and 60%(27/45),respectively.The positive rates of cyclinD1 and CDK4 were significantly higher in the cases which pathological classification was histological grade I and without metastasis than that pathological classification was histological grade Ⅲ and with metastasis(histological grade,P＜0.05;metastatic state,P＞0.05).The closely positive correlation was found between the expressions of cyclinD1 and CDK4 in malignant bone tumors.Conclusions:The expressions of cyclinD1 and CDK4might be related to the biological behaviors and prognosis of malignant bone tumors.Abnormality in the regulatory pathway of cyclinDl/CDK4 might be involved in molecular mechanism of malignant bone tumors.The detections of cyclinD1 and CDK4 in benign lesions might have important clinical significance in the prevention and early-stage discovery of malignant bone tumors.