CAJ | 학술논문

目的探讨人胚胎胰腺中干细胞特异性标志巢蛋白(Nestin) 的表达及阳性细胞的分布. 方法用连续切片免疫组织化学染色方法检测16周胎龄人胚胎胰腺中巢蛋白、胰岛素、胰升糖素和导管上皮标志细胞角蛋白19(CK19)的表达及分布. 结果 16周人胚胎胰腺中可检测到巢蛋白阳性细胞,分布于胰岛以及胰导管和腺泡结构中,并且以腺泡结构和小导管中的数目为多.部分胰岛素和CK19染色强阳性的细胞中巢蛋白染色为弱阳性,胰升糖素强阳性的部位则未见巢蛋白染色阳性的细胞. 结论人胚胎胰腺中存在巢蛋白表达的细胞;巢蛋白阳性细胞可能是胰腺组织中一个特殊的细胞亚群,代表了一群未成熟细胞.
목적탐토인배태이선중간세포특이성표지소단백(Nestin) 적표체급양성세포적분포. 방법용련속절편면역조직화학염색방법검측16주태령인배태이선중소단백、이도소、이승당소화도관상피표지세포각단백19(CK19)적표체급분포. 결과 16주인배태이선중가검측도소단백양성세포,분포우이도이급이도관화선포결구중,병차이선포결구화소도관중적수목위다.부분이도소화CK19염색강양성적세포중소단백염색위약양성,이승당소강양성적부위칙미견소단백염색양성적세포. 결론인배태이선중존재소단백표체적세포;소단백양성세포가능시이선조직중일개특수적세포아군,대표료일군미성숙세포.
Objective To investigate whether nestin-expressing cells are present in human fetal pancreas and the cells distribution. Methods Immuno- histochemical staining was used to determine the expression and distribution of nestin, insulin, glucagon and cytokeratin 19 (CK19) in the consecutive sections prepared from human fetal pancreas at 16 weeks of gestation. Results There were nestin-expressing cells in the 16-week human fetal pancreas. These cells resided in the islets of Langerhans, pancreatic ductal and acinic structures, and were the most abundant in both acini and small ducts. Some of the insulin and CK19 positively staining cells were positive for nestin expression. There were no nestin positive cells within the areas where glucagon-expressing cells resided. Conclusion The cells expressing nestin, a marker for pancreatic stem cells, may be a subpopulation of immature cells in the human fetal pancreas.