CAJ | 학술논문

目的研究 HBV反义全基因真核表达载体在抗 HBV基因治疗的可能性。方法采用基因重组技术，将 HBV全基因片段反向插入真核细胞表达载体 pBK－ CMV克隆位点中，通过 FuGENE TM6转染试剂，将重组体转染人肝癌细胞系 SMM C－ 7721细胞。结果经 RT－ PCR表明，重组体导入的人肝癌细胞内有 HBV反义 RNA转录表达，且重组体导入对细胞生长无明显影响。结论 HBV全基因真核细胞表达载体在 SMMC－ 7721细胞中能转录表达反义 RNA，因而有可能利用反义技术和转基因方法进行 HBV全基因反义 RNA抗 HBV研究。
목적연구 HBV반의전기인진핵표체재체재항 HBV기인치료적가능성。방법채용기인중조기술，장 HBV전기인편단반향삽입진핵세포표체재체 pBK－ CMV극륭위점중，통과 FuGENE TM6전염시제，장중조체전염인간암세포계 SMM C－ 7721세포。결과경 RT－ PCR표명，중조체도입적인간암세포내유 HBV반의 RNA전록표체，차중조체도입대세포생장무명현영향。결론 HBV전기인진핵세포표체재체재 SMMC－ 7721세포중능전록표체반의 RNA，인이유가능이용반의기술화전기인방법진행 HBV전기인반의 RNA항 HBV연구。
Objective To probe the way by which the antisense gene of entire HBV genome can be transferred and transcripted in hepatoma cells to inhibit HBV replication and expression. Methods Using molecular clone technique, a mammalian vector that carried antisense gene of entire HBV genome was constructed, and it was transfected into a hepatoma cell line, SMMC— 7721 with FUGENETM 6 transfaction reagent. Results RT-PCR analysis showed that the antisense gene of entire HBV genome can be successfully transferred and transcripted in target cells. Conclusion The vector may be potentially useful for anti－HBV gene therapy.