中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2001年
5期
388-391
,共4页
邓宇斌%李树浓%周平坤%Magali T
鄧宇斌%李樹濃%週平坤%Magali T
산우빈%리수농%주평곤%Magali T
范科尼贫血%辐射,电离%基因表达
範科尼貧血%輻射,電離%基因錶達
범과니빈혈%복사,전리%기인표체
目的：研究正常人外周血单核细胞在UV或γ辐射后hHR21sp基因的转录表达，进一步分析hHR21sp基因在fanconis anemia(FA)单核细胞和造血干细胞的转录表达水平及发病机制中的作用。方法：对正常人外周血单核细胞在UV或γ辐射后不同时间提取细胞总RNA，通过RT-PCR、 southern杂交、以β-actin为内参照放射影像对hHR21sp基因的转录表达水平进行检测，进一步检测FA病人外周血单核细胞和骨髓造血细胞hHR21sp基因的表达水平。结果：正常外周血单核细胞在UV或γ-辐射后不同时间hHR21sp基因转录表达都有所变化。正常人外周血单核细胞与对照组比较在80 J/m２UV辐射hHR21sp表达于3 h开始增加；在6 h表达水平最高，与正常对照有2倍多差异，而在9 h表达有所降低；而γ-辐射5Gy辐射6 h增加明显，在9 h后有所降低与正常对照有2倍差异。检测发现FA病人单核细胞与正常人外周血单核细胞hHR21sp基因表达无明显差异；但FA病人造血干细胞与正常组骨髓细胞相比hHR21sp基因表达降低显著，有1倍多差异。结论：实验结果表明hHR21sp在FA病人造血干细胞表达缺陷，提示hHR21sp基因表达降低可影响FA病人造血干细胞和DNA双链断裂修复功能是FA发病机制之一。
目的：研究正常人外週血單覈細胞在UV或γ輻射後hHR21sp基因的轉錄錶達，進一步分析hHR21sp基因在fanconis anemia(FA)單覈細胞和造血榦細胞的轉錄錶達水平及髮病機製中的作用。方法：對正常人外週血單覈細胞在UV或γ輻射後不同時間提取細胞總RNA，通過RT-PCR、 southern雜交、以β-actin為內參照放射影像對hHR21sp基因的轉錄錶達水平進行檢測，進一步檢測FA病人外週血單覈細胞和骨髓造血細胞hHR21sp基因的錶達水平。結果：正常外週血單覈細胞在UV或γ-輻射後不同時間hHR21sp基因轉錄錶達都有所變化。正常人外週血單覈細胞與對照組比較在80 J/m２UV輻射hHR21sp錶達于3 h開始增加；在6 h錶達水平最高，與正常對照有2倍多差異，而在9 h錶達有所降低；而γ-輻射5Gy輻射6 h增加明顯，在9 h後有所降低與正常對照有2倍差異。檢測髮現FA病人單覈細胞與正常人外週血單覈細胞hHR21sp基因錶達無明顯差異；但FA病人造血榦細胞與正常組骨髓細胞相比hHR21sp基因錶達降低顯著，有1倍多差異。結論：實驗結果錶明hHR21sp在FA病人造血榦細胞錶達缺陷，提示hHR21sp基因錶達降低可影響FA病人造血榦細胞和DNA雙鏈斷裂脩複功能是FA髮病機製之一。
목적：연구정상인외주혈단핵세포재UV혹γ복사후hHR21sp기인적전록표체，진일보분석hHR21sp기인재fanconis anemia(FA)단핵세포화조혈간세포적전록표체수평급발병궤제중적작용。방법：대정상인외주혈단핵세포재UV혹γ복사후불동시간제취세포총RNA，통과RT-PCR、 southern잡교、이β-actin위내삼조방사영상대hHR21sp기인적전록표체수평진행검측，진일보검측FA병인외주혈단핵세포화골수조혈세포hHR21sp기인적표체수평。결과：정상외주혈단핵세포재UV혹γ-복사후불동시간hHR21sp기인전록표체도유소변화。정상인외주혈단핵세포여대조조비교재80 J/m２UV복사hHR21sp표체우3 h개시증가；재6 h표체수평최고，여정상대조유2배다차이，이재9 h표체유소강저；이γ-복사5Gy복사6 h증가명현，재9 h후유소강저여정상대조유2배차이。검측발현FA병인단핵세포여정상인외주혈단핵세포hHR21sp기인표체무명현차이；단FA병인조혈간세포여정상조골수세포상비hHR21sp기인표체강저현저，유1배다차이。결론：실험결과표명hHR21sp재FA병인조혈간세포표체결함，제시hHR21sp기인표체강저가영향FA병인조혈간세포화DNA쌍련단렬수복공능시FA발병궤제지일。
AIM:To detect the expression of hHR21sp gene in normal human peripheral blood cells and in hemapoietic cell from fanconi anemia(FA) patients bone marrow after exposure to UV and gamma-irradiation.METHODS:RNA of FA hemapoietic cell and normal peripheral blood cells were harvested after UV and gamma irradiation. hHR21sp gene expression was analyzed using RT-PCR, southern blot hybridization, phosphoimmage autoradiogram.RESULTS:The expression of hHR21sp gene in PBL varied with the time of UV gamma-irradiation and increased obviously six hours after 80 J/M２ UV-or 5 Gy gamma-irradiation. However, the expression was down-regulated in FA hemapoietic cell from FA patients indicating that hHR21sp expression is something related to the ability of cells to repair DNA double strand break.CONCLUSION:The expression of hHR21sp down-regulated in the FA syndrome and this might be involved in the pathogenesis of the FA.
