CAJ | 학술논문

目的　了解子宫内膜异位症（内异症）患者腹腔液中自然杀伤细胞(NK细胞)活性以及白细胞介素-6(IL-6)、白细胞介素-8（IL-8)、肿瘤坏死因子α（TNF-α）水平的变化及其意义。方法　从32例内异症患者（轻度组13例，为Ⅰ，Ⅱ期，重度组19例，为Ⅲ，Ⅳ期）和 19例对照者（对照组，为行输卵管结扎术和吻合术患者)的腹腔液中获取自然杀伤细胞，同时收集上清液。用放射性同位素法测定NK细胞活性，用酶联免疫吸附法测定IL-6，IL-8和TNF-α的水平。结果　内异症轻度组和重度组NK细胞活性均显著低于对照组(P＜0.01)，重度组显著低于轻度组(P＜0.01)，轻度组和重度组IL-6， IL-8及TNF-α 水平均明显高于对照组，差异有极显著性（P＜0.01）；重度组显著高于轻度组(P＜0.01)。结论　内异症患者腹腔液中免疫功能紊乱，NK细胞活性受损，细胞因子 IL-6，IL-8，TNF-α产生过量，且NK细胞活性受损程度及产生细胞因子的量随疾病分期的增加而增高，可能共同参与内异症的发病过程。
목적　료해자궁내막이위증（내이증）환자복강액중자연살상세포(NK세포)활성이급백세포개소-6(IL-6)、백세포개소-8（IL-8)、종류배사인자α（TNF-α）수평적변화급기의의。방법　종32례내이증환자（경도조13례，위Ⅰ，Ⅱ기，중도조19례，위Ⅲ，Ⅳ기）화 19례대조자（대조조，위행수란관결찰술화문합술환자)적복강액중획취자연살상세포，동시수집상청액。용방사성동위소법측정NK세포활성，용매련면역흡부법측정IL-6，IL-8화TNF-α적수평。결과　내이증경도조화중도조NK세포활성균현저저우대조조(P＜0.01)，중도조현저저우경도조(P＜0.01)， 경도조화중도조IL-6， IL-8급TNF-α 수평균명현고우대조조，차이유겁현저성（P＜0.01）；중도조현저고우경도조(P＜0.01)。결론　내이증환자복강액중면역공능문란，NK세포활성수손，세포인자 IL-6，IL-8，TNF-α산생과량，차NK세포활성수손정도급산생세포인자적량수질병분기적증가이증고，가능공동삼여내이증적발병과정。
Objective 　To study the change and significance of natural killer cell (NK cell)activity, interleukin-6(IL-6),interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) levels in peritoneal fluid of patients with endometriosis. Methods 32 patients with endometriosis were divided into two groups(mild group: 13,stageⅠ or Ⅱ; severe group: 19,stage Ⅲ or Ⅳ ). 21 controls were also included. The NK cell activity and levels of IL-6,IL-8 and TNF-α in peritoneal fluid were measured by radioisotope method or enzyme linked immunosorbent assays. Results The NK cell activity in peritoneal fluid from two endometriosis groups was significantly lower than that in controls (P＜0.01), and NK cell activity of severe group was significantly lower than that of mild group.The concentrations of IL-6,IL-8 and TNF-α were significantly higher in two endometriosis groups than those in controls (P＜0.01), with significant differences between two endometriosis groups (P＜0.01).Conclusions The immune function of peritoneal fluid of patients with endometriosis is impaired. The NK cell activity is lower,the levels of IL-6,IL-8 and TNF-α are higher. The clinical stage of endometriosis is positively correlated to cytokine level and negatively correlated to NK cell activily. They may participate in the pathogenesis of endometriosis.