CAJ | 학술논문

用根癌农杆菌（Agrobacterium tumefaciens）菌株C58感染栝楼（Trichosanthes kirilowii Maxim.）无菌苗诱导冠瘿瘤，获得其冠瘿组织；栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，纸电泳检测结果表明其合成了冠瘿碱，表明Ti质粒转化成功。栝楼冠瘿组织在MS培养基上形成完整植株，移栽后良好生长。
용근암농간균（Agrobacterium tumefaciens）균주C58감염괄루（Trichosanthes kirilowii Maxim.）무균묘유도관영류，획득기관영조직；괄루관영조직경제균후능재무격소적MS배양기상량호생장，지전영검측결과표명기합성료관영감，표명Ti질립전화성공。괄루관영조직재MS배양기상형성완정식주，이재후량호생장。
Crown galls were induced by direct infection of sterilized seedlings of Trichosanthes kirilowii Maxim. with Agrobacterium tumefaciens C58. Transformation of Ti in C58 was inspected by high_pressure_paper electrophoresis; the detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully. The crown tissues produced plantlets on MS medium and the plantlets were successfully transplanted and grew well.